首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BGLAP含量。BGLAP即骨钙素，是骨组织中含量丰富的非胶原蛋白。其背景在于它是成骨细胞特异性分泌的，反映骨代谢状态。研究机制方面，它不仅参与骨矿化和骨转换，还在能量代谢、生殖等系统有调节作用，为多领域疾病研究提供潜在靶点。试剂盒检测范围为1.25 ng/mL-80 ng/mL，常用于骨代谢调控机制、骨质疏松症模型评估及骨骼相关药物开发等研究。适用于体外分析骨组织工程材料对成骨细胞功能的影响、疾病模型中骨代谢动态监测、以及骨相关生物标志物的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写：BGLAP
Uniprot No.：P39056
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-90 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 90-94 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.7802.7012.7412.641402.1242.0562.0901.990201.2661.2341.2501.150100.6670.6210.6440.54450.3530.3350.3440.2442.50.2620.2490.2560.1561.250.2190.2080.2140.11400.1020.0980.100 | ||||||
货期：3-5 working days
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