| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CIA1; WDR39 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CIAO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CIAO1抗体的参考文献(文献信息为模拟示例，实际文献需根据具体数据库检索验证)：
1. **文献名称**：*"CIAO1 is a component of the mitochondrial iron-sulfur cluster assembly machinery"*
**作者**：Stehling O, et al.
**摘要**：本研究利用CIAO1特异性抗体，通过Western blot和免疫荧光技术，证实CIAO1参与线粒体铁硫簇(Fe-S)组装通路，并揭示其与ISC复合物的相互作用。
2. **文献名称**：*"CIAO1 interacts with BRCA1 and modulates DNA damage response"*
**作者**：Kim H, et al.
**摘要**：通过免疫共沉淀(Co-IP)结合CIAO1抗体，发现CIAO1与BRCA1蛋白存在直接互作，并证明其在DNA损伤修复中的调控功能，为癌症治疗提供潜在靶点。
3. **文献名称**：*"Antibody-based profiling of CIAO1 expression in human cancers"*
**作者**：Smith JL, et al.
**摘要**：开发并验证了一种高特异性CIAO1单克隆抗体，用于免疫组化分析，发现CIAO1在多种实体瘤中异常高表达，提示其作为肿瘤标志物的潜力。
如需具体文献，建议通过PubMed或Google Scholar检索关键词“CIAO1 antibody”或“CIAO1 immunodetection”获取最新研究。
The CIAO1 (Cytosolic Iron-Sulfur Assembly Component 1) antibody is a tool used to study the CIAO1 protein, a key player in the cytosolic iron-sulfur cluster (Fe-S) assembly pathway. Fe-S clusters are essential cofactors for numerous enzymes involved in DNA repair, redox reactions, and metabolic processes. CIAO1. part of the multi-protein CIA complex, facilitates the maturation and transfer of Fe-S clusters to target apoproteins in the cytosol and nucleus.
Research on CIAO1 has gained attention due to its role in maintaining genomic stability and cellular iron homeostasis. Dysregulation of Fe-S biogenesis is linked to diseases such as cancer, neurodegenerative disorders, and anemia. The CIAO1 antibody enables detection and quantification of CIAO1 expression in various experimental setups, including Western blotting, immunofluorescence, and immunohistochemistry. It has been particularly useful in studies exploring DNA damage response mechanisms, mitochondrial dysfunction, and cancer biology, where altered Fe-S cluster metabolism may drive disease progression.
Commercial CIAO1 antibodies are typically raised against specific epitopes of human CIAO1. with validation in multiple cell lines and tissues. Researchers rely on this reagent to investigate how CIAO1 interacts with other Fe-S assembly components (e.g., CIA2B, MMS19) or client proteins, offering insights into cellular adaptation to oxidative stress or iron scarcity. Its applications extend to both basic research and clinical studies aiming to dissect Fe-S-related pathologies.
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