| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PBK |
| Uniprot No | Q96KB5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PBK(PDZ-binding kinase)重组蛋白的3篇示例参考文献(注：文献信息为示例性内容，非真实存在)：
1. **文献名称**: "Recombinant PBK Protein Expression and Its Role in Mitotic Progression"
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化重组PBK蛋白，通过体外激酶实验证实其磷酸化组蛋白H3的功能，揭示了PBK在细胞有丝分裂中的调控机制。
2. **文献名称**: "Structural Characterization of PBK Kinase Domain and Its Inhibitor Screening"
**作者**: Kim S, Park JH.
**摘要**: 采用X射线晶体学解析了PBK激酶结构域的三维结构，并基于此筛选出小分子抑制剂，为靶向PBK的癌症治疗提供了结构基础。
3. **文献名称**: "PBK/TOPK Recombinant Protein Enhances DNA Damage Response in Glioblastoma Cells"
**作者**: Chen R, et al.
**摘要**: 通过哺乳动物细胞表达系统制备重组PBK蛋白，证明其通过激活ATM/ATR通路增强胶质母细胞瘤的DNA损伤修复能力，提示其在肿瘤耐药性中的潜在作用。
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**注**：以上为模拟参考文献，实际研究中建议通过PubMed或Web of Science检索真实文献(关键词：PBK/TOPK, recombinant protein, kinase)。真实文献中常涉及PBK在癌症、干细胞分化及信号通路中的功能研究。
**Background of PBK Recombinant Protein**
PBK (PDZ-binding kinase), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase belonging to the mitogen-activated protein kinase kinase (MAPKK) family. It plays a critical role in regulating cell cycle progression, DNA damage response, and mitotic processes. PBK/TOPK is highly expressed in rapidly proliferating cells, including cancer cells, where it promotes tumor growth, invasion, and metastasis by modulating pathways such as the ERK/MAPK and NF-κB signaling cascades. Its interaction with key proteins like p53 and survivin further underscores its importance in apoptosis evasion and genomic instability.
Recombinant PBK protein is engineered using biotechnological methods, often expressed in *E. coli* or mammalian systems to ensure proper post-translational modifications. The protein is purified via affinity chromatography, retaining functional activity for experimental applications. Researchers utilize PBK recombinant protein to study its enzymatic kinetics, substrate interactions, and regulatory mechanisms in vitro. It also serves as a vital tool for screening inhibitors in drug discovery, particularly in oncology, given its association with chemoresistance and poor prognosis in cancers like glioblastoma, breast, and colorectal carcinoma.
The development of PBK recombinant protein has accelerated structural studies, epitope mapping, and antibody production, enhancing understanding of its role in diseases. Its potential as a therapeutic target continues to drive interest in designing small-molecule inhibitors or biologics to disrupt PBK-mediated oncogenic pathways.
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