| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAX9 |
| Uniprot No | P55771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEPAFGE VNQLGGVFVN GRPLPNAIRL RIVELAQLGI RPCDISRQLR VSHGCVSKIL ARYNETGSIL PGAIGGSKPR VTTPTVVKHI RTYKQRDPGI FAWEIRDRLL ADGVCDKYNV PSVSSISRIL RNKIGNLAQQ GHYDSYKQHQ PTPQPALPYN HIYSYPSPIT AAAAKVPTPP GVPAIPGSVA MPRTWPSSHS VTDILGIRSI TDQVSDSSPY HSPKVEEWSS LGRNNFPAAA PHAVNGLEKG ALEQEAKYGQ APNGLPAVGS FVSASSMAPY PTPAQVSPYM TYSAAPSGYV AGHGWQHAGG TSLSPHNCDI PASLAFKGMQ AAREGSHSVT ASAL |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAX9重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
1. **文献名称**：*Functional analysis of PAX9 recombinant protein in tooth development*
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化PAX9重组蛋白，验证其DNA结合活性，揭示其在牙齿发育中调控釉质蛋白基因的分子机制。
2. **文献名称**：*PAX9 recombinant protein promotes osteogenic differentiation in vitro*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞表达PAX9重组蛋白，发现其通过激活BMP/Smad信号通路增强间充质干细胞的成骨分化，为骨再生研究提供新靶点。
3. **文献名称**：*Structural characterization of PAX9-DNA interaction using recombinant protein*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析PAX9重组蛋白与靶DNA复合物的三维结构，阐明其保守的配对结构域结合基序，为先天性缺牙症突变分析提供结构基础。
*注：实际文献需通过PubMed等数据库检索确认。*
**Background of PAX9 Recombinant Protein**
PAX9. a member of the PAX (paired box) family of transcription factors, plays a critical role in embryonic development, particularly in organogenesis and tissue patterning. It is encoded by the *PAX9* gene, located on human chromosome 14. and is characterized by a conserved DNA-binding paired domain that regulates the expression of downstream target genes. PAX9 is essential for the development of multiple structures, including teeth, limbs, and the thymus, as well as craniofacial and nervous systems. Mutations in *PAX9* are strongly associated with congenital disorders such as oligodontia (tooth agenesis) and syndromes involving ectodermal dysplasia.
Recombinant PAX9 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and functionality. This engineered protein retains the DNA-binding and transcriptional regulatory properties of native PAX9. making it a valuable tool for studying molecular mechanisms in developmental biology and disease. Researchers utilize PAX9 recombinant protein to investigate its interactions with DNA motifs, co-regulatory proteins, and signaling pathways (e.g., BMP, FGF, and Wnt) during organ formation.
In regenerative medicine and disease modeling, PAX9 recombinant protein aids in exploring potential therapeutic strategies for congenital anomalies or tissue regeneration. Its application extends to in vitro assays, such as electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP), to map binding sites and gene regulatory networks. Additionally, it serves as a reference in drug screening to identify compounds that modulate PAX9 activity.
Overall, PAX9 recombinant protein bridges fundamental research and translational applications, enhancing our understanding of developmental disorders and paving the way for innovative biomedical interventions.
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