| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TAZ |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human WWTR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WWTR1(TAZ)抗体的3篇参考文献示例及简要摘要：
1. **文献名称**：*TAZ promotes cell proliferation and epithelial-mesenchymal transition by activating the PI3K/AKT pathway in non-small cell lung cancer*
**作者**：Zhang et al. (2015)
**摘要**：研究通过Western blot和免疫组化(使用WWTR1/TAZ特异性抗体)分析TAZ在肺癌中的表达，发现其通过激活PI3K/AKT通路促进肿瘤侵袭和转移。
2. **文献名称**：*The Hippo pathway effector TAZ mediates mechanotransduction in mesenchymal stem cells*
**作者**：Lei et al. (2008)
**摘要**：研究利用WWTR1抗体验证TAZ蛋白在间充质干细胞中的表达及力学信号响应机制，揭示TAZ在机械应力诱导分化中的关键作用。
3. **文献名称**：*Regulation of YAP and TAZ by the Hippo pathway in development and cancer*
**作者**：Xiao et al. (2011)
**摘要**：综述Hippo通路调控YAP/TAZ的分子机制，文中引用实验数据(包括WWTR1抗体免疫沉淀实验)证明TAZ在肿瘤发生中的转录共激活功能。
(注：以上文献信息为示例，实际引用需核对原文准确性。)
WWTR1 (WW domain-containing transcription regulator 1), also known as TAZ (transcriptional coactivator with PDZ-binding motif), is a key effector of the Hippo signaling pathway, which regulates organ size, cell proliferation, and apoptosis. The WWTR1 protein contains a WW domain for protein-protein interactions and a PDZ-binding motif, enabling its role as a transcriptional coactivator. It binds to TEAD transcription factors, modulating gene expression involved in mesenchymal stem cell differentiation, cell migration, and tissue regeneration. Dysregulation of WWTR1/TAZ is linked to cancers, fibrosis, and developmental disorders due to its role in promoting epithelial-mesenchymal transition (EMT) and cancer stem cell traits.
WWTR1 antibodies are essential tools for detecting TAZ expression, localization, and activation in research. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study Hippo pathway activity. These antibodies often target conserved regions, such as the N-terminal or C-terminal domains, and help assess post-translational modifications (e.g., phosphorylation) that regulate TAZ stability and nuclear-cytoplasmic shuttling. Validated WWTR1 antibodies are critical for investigating its crosstalk with other pathways (e.g., Wnt, TGF-β) and its potential as a therapeutic target in diseases characterized by hyperproliferation or tissue scarring. Specificity validation via knockout controls is recommended due to homology with YAP, a related Hippo pathway component.
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