| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CPN4; COPN4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CPNE4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于公开文献的模拟示例(仅供参考，具体文献需查阅数据库)：
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1. **文献名称**: *"CPNE4 as a novel prognostic marker in prostate cancer: immunohistochemical analysis"*
**作者**: Smith J, et al.
**摘要**: 该研究通过免疫组化分析发现CPNE4在晚期前列腺癌组织中高表达，并与患者预后不良相关。使用特异性CPNE4抗体证实其蛋白水平与肿瘤侵袭性正相关，提示其作为潜在生物标志物的价值。
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2. **文献名称**: *"Role of CPNE4 in Alzheimer's disease pathogenesis"*
**作者**: Lee H, et al.
**摘要**: 研究利用CPNE4抗体检测阿尔茨海默病模型小鼠脑组织，发现CPNE4在淀粉样斑块周围神经元中表达上调，可能通过调节钙信号通路加剧神经退行性病变。
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3. **文献名称**: *"CPNE4 regulates epithelial-mesenchymal transition in lung adenocarcinoma"*
**作者**: Wang Y, et al.
**摘要**: 通过Western blot和免疫荧光技术(使用CPNE4抗体)证实，CPNE4通过调控TGF-β通路促进肺癌细胞EMT过程，其表达水平与转移风险显著相关。
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4. **文献名称**: *"Developmental expression pattern of CPNE4 in the murine brain"*
**作者**: Garcia R, et al.
**摘要**: 研究利用CPNE4抗体进行小鼠脑组织染色，发现CPNE4在胚胎期海马及皮层区域特异性高表达，提示其在神经发育中的潜在作用。
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**注意事项**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“CPNE4 antibody”或“CPNE4 protein expression”检索，并验证实验方法(如抗体货号、应用场景)。
The CPNE4 antibody targets Copine-4 (CPNE4), a calcium-dependent phospholipid-binding protein belonging to the copine family, which is evolutionarily conserved and involved in membrane trafficking, signal transduction, and cellular adhesion. CPNE4 is encoded by the CPNE4 gene located on human chromosome 3q21.3 and is expressed in various tissues, including the brain, lung, and testis. It plays roles in regulating intracellular pathways, such as NF-κB signaling, and has been implicated in neuronal development, synaptic plasticity, and cancer progression. Studies suggest CPNE4 may act as a tumor suppressor or promoter depending on context, with altered expression observed in gliomas, prostate cancer, and other malignancies.
In neuroscience, CPNE4 is associated with neurodegenerative diseases, potentially influencing Aβ aggregation in Alzheimer’s disease. Its calcium-dependent membrane-binding ability allows it to bridge cytoplasmic proteins with membrane phospholipids, modulating vesicle transport and receptor signaling. CPNE4 antibodies are essential tools for detecting protein expression, localization, and interaction partners via techniques like Western blot, immunohistochemistry, or immunofluorescence. Researchers utilize these antibodies to explore CPNE4's functional mechanisms in health and disease, though variability in antibody specificity across commercial sources requires careful validation for experimental reliability. Ongoing research aims to clarify its dual roles in cancer and neurodegeneration.
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