| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CPT1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于CPT1B抗体的参考文献示例(内容基于模拟生成，非真实文献)：
1. **名称**：*"Characterization of CPT1B antibody specificity in skeletal muscle metabolism"*
**作者**：Lee, J. et al. (2018)
**摘要**：本研究验证了一种高特异性CPT1B抗体在人和小鼠骨骼肌组织中的应用，通过免疫印迹和免疫组化证实其识别线粒体膜上的CPT1B蛋白，并揭示其在脂肪酸氧化调控中的作用。
2. **名称**：*"CPT1B deficiency in cardiac ischemia-reperfusion injury: Insights from antibody-based detection"*
**作者**：Smith, R. & Zhang, H. (2020)
**摘要**：利用CPT1B特异性抗体，研究发现心脏缺血再灌注损伤模型中CPT1B表达显著下调，提示其可能通过影响线粒体脂肪酸代谢加重心肌损伤。
3. **名称**：*"Development of a novel monoclonal antibody for human CPT1B and its application in obesity research"*
**作者**：Garcia, M. et al. (2021)
**摘要**：报道了一种新型单克隆CPT1B抗体的开发，并在肥胖患者肌肉组织中验证其应用，发现CPT1B蛋白水平与胰岛素抵抗呈负相关。
4. **名称**：*"CPT1B antibody reveals exercise-induced mitochondrial adaptation in skeletal muscle"*
**作者**：Wang, Y. et al. (2019)
**摘要**：通过CPT1B抗体检测，证明长期有氧运动上调骨骼肌线粒体CPT1B表达，增强脂肪酸氧化能力，为代谢疾病治疗提供依据。
(注：以上文献为示例性内容，实际引用请查询PubMed或SciFinder等数据库获取真实文献。)
The CPT1B antibody is a crucial tool for studying carnitine palmitoyltransferase 1B (CPT1B), a mitochondrial enzyme central to fatty acid metabolism. CPT1B, one of three CPT1 isoforms (CPT1A, CPT1B, CPT1C), is predominantly expressed in skeletal muscle and heart tissues. It catalyzes the rate-limiting step of fatty acid β-oxidation by transferring long-chain fatty acids into mitochondria, a process vital for energy production during fasting or prolonged exercise. Dysregulation of CPT1B is linked to metabolic disorders, including obesity, insulin resistance, and cardiovascular diseases, making it a target for metabolic research.
CPT1B antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and tissue-specific distribution. They help investigate CPT1B’s role in metabolic adaptations, such as exercise-induced changes or dietary interventions. Researchers also employ these antibodies to explore pathological conditions, such as cancer (where altered lipid metabolism supports tumor growth) or muscle-related metabolic syndromes. Specificity validation (e.g., knockout controls) is critical due to structural similarities among CPT1 isoforms. Commercial CPT1B antibodies are typically raised against unique epitopes in the N-terminal or C-terminal regions, with host species varying (rabbit, mouse). Their applications extend to preclinical studies targeting CPT1B for therapeutic modulation in metabolic diseases.
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