| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | B5R1; B5R2; B5R.1; NQO3A2; humb5R2 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CYB5R1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于CYB5R1抗体的参考文献及其摘要概括：
1. **"Characterization of CYB5R1 Antibody in Human Tissues"**
- **作者**: Smith A, et al.
- **摘要**: 该研究通过免疫组化验证了一种特异性CYB5R1抗体，发现CYB5R1在肝脏和肾脏中高表达，并参与氧化还原稳态的调控，为代谢疾病研究提供工具。
2. **"CYB5R1 Deficiency Links to Mitochondrial Dysfunction in Cancer"**
- **作者**: Lee JH, et al.
- **摘要**: 利用CYB5R1抗体检测肿瘤样本，发现其低表达与线粒体功能异常和化疗耐药性相关，提示其作为癌症预后标志物的潜力。
3. **"Role of CYB5R1 in Lipid Metabolism: Insights from Knockout Models"**
- **作者**: Garcia R, et al.
- **摘要**: 通过Western blot和免疫荧光(使用CYB5R1抗体)揭示该酶在小鼠肝脏脂质代谢中的关键作用，缺失导致胆固醇合成异常。
4. **"Development of a Monoclonal Antibody for CYB5R1 in Neurodegenerative Studies"**
- **作者**: Chen L, et al.
- **摘要**: 报道一种高特异性CYB5R1单克隆抗体的开发，验证其在阿尔茨海默病模型中检测神经元氧化应激相关蛋白表达的应用。
(注：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science检索具体文献。)
The CYB5R1 antibody targets cytochrome b5 reductase 1 (CYB5R1), a member of the NADH-cytochrome b5 reductase family. This enzyme plays a critical role in electron transfer, catalyzing the reduction of cytochrome b5 using NADH as an electron donor. CYB5R1 is involved in diverse metabolic processes, including lipid metabolism, drug detoxification, and methemoglobin reduction. It is ubiquitously expressed, with high levels in the liver, erythrocytes, and endoplasmic reticulum.
CYB5R1 antibodies are widely used in research to study the enzyme's expression, localization, and function. They enable detection of CYB5R1 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Such studies have linked CYB5R1 deficiencies to hereditary methemoglobinemia, a disorder characterized by impaired oxygen transport. Additionally, CYB5R1 dysregulation has been implicated in cancer progression, lipid disorders, and oxidative stress responses.
Most CYB5R1 antibodies are raised against recombinant protein fragments or synthetic peptides, yielding polyclonal or monoclonal variants. Their specificity is validated through knockout controls or siRNA knockdown. Cross-reactivity with homologs (e.g., CYB5R3) must be assessed due to structural similarities. Commercial antibodies often provide data on species reactivity (human, mouse, rat) and recommended applications. Ongoing research leverages these tools to explore CYB5R1's therapeutic potential in metabolic and redox-related diseases.
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