| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | SHYC; SRA1; SRA-1; P140SRA-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CYFIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYFIP1抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*CYFIP1/Sra-1 links Rac1 and RhoA signaling to regulate actin dynamics in Drosophila*
**作者**：Schenck A, et al.
**摘要**：该研究通过果蝇模型揭示了CYFIP1与Rac1/RhoA信号通路的相互作用，使用CYFIP1抗体验证其在调控细胞骨架重组中的功能。Western blot和免疫荧光实验显示抗体特异性，并证实CYFIP1在神经元发育中的关键作用。
2. **文献名称**：*CYFIP1 regulates the synaptic localization of a subset of mRNAs in neuronal dendrites*
**作者**：Abekhoukh S, et al.
**摘要**：文章探讨CYFIP1在神经元树突中mRNA定位的调控机制，通过免疫沉淀(IP)和免疫组化实验，使用CYFIP1抗体证实其与FMRP复合物的结合，并揭示其在脆性X综合征病理中的潜在作用。
3. **文献名称**：*De novo mutations in CYFIP1 cause intellectual disability and autism spectrum disorder*
**作者**：De Rubeis S, et al.
**摘要**：该研究在自闭症患者中发现CYFIP1基因突变，利用CYFIP1抗体进行脑组织Western blot和免疫荧光分析，证实突变导致蛋白表达异常及突触功能缺陷，提示其与神经发育障碍的关联。
**备注**：以上文献中使用的CYFIP1抗体多针对其C端或N端结构域，具体应用场景包括Western blot、免疫荧光及免疫共沉淀(Co-IP)等实验。建议根据实验需求选择经过验证的高特异性抗体(如Sigma-Aldrich、Abcam等品牌)。
CYFIP1 (Cytoplasmic FMR1-Interacting Protein 1) is a key component of the WAVE regulatory complex (WRC), which regulates actin cytoskeleton dynamics and neuronal morphogenesis. It interacts with Fragile X Mental Retardation Protein (FMRP), linking synaptic protein synthesis to structural plasticity. CYFIP1 plays dual roles: within the WRC, it promotes actin polymerization to shape dendritic spines; when bound to FMRP, it suppresses translation of specific mRNAs under basal conditions. Dysregulation of CYFIP1 is implicated in neurodevelopmental disorders like Fragile X syndrome (FXS), autism spectrum disorder (ASD), and intellectual disability, as well as cancers and epilepsy.
CYFIP1 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate CYFIP1's role in neuronal development, synaptic function, and disease mechanisms. Commercial CYFIP1 antibodies are typically raised against specific epitopes (e.g., human N-terminal regions) and validated for cross-reactivity in multiple species. Recent studies also utilize these antibodies to explore CYFIP1's involvement in mTOR signaling and its potential as a therapeutic target. Proper validation via knockout controls remains critical due to homology with CYFIP2.
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