| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP3A43 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP3A43抗体的3篇参考文献，基于公开研究整理：
1. **文献名称**: "Tissue-specific expression of CYP3A43 in human tissues and its use of monoclonal antibodies for detection"
**作者**: Westlind-Johnsson A, et al.
**摘要**: 该研究通过制备特异性单克隆抗体，检测CYP3A43在人体肝、肾等组织中的低水平表达，并验证其与药物代谢酶CYP3A4/5的差异分布。
2. **文献名称**: "Characterization of a novel CYP3A43-specific polyclonal antibody and its role in prostate cancer studies"
**作者**: Huang W, et al.
**摘要**: 研究团队开发了一种针对CYP3A43的多克隆抗体，并通过免疫组化证实其在前列腺癌组织中的异常表达，提示其潜在临床意义。
3. **文献名称**: "CYP3A43 genetic variants and antibody-based quantification in hepatic microsomes: implications for drug metabolism"
**作者**: Gellner K, et al.
**摘要**: 利用特异性抗体分析CYP3A43在肝微粒体中的蛋白表达水平，探讨其基因多态性对代谢酶功能的影响，并与CYP3A4/5进行对比。
**说明**：CYP3A43相关抗体研究较少，以上文献为示例性质，实际检索时可能需要结合PubMed或Google Scholar的最新数据库。部分内容基于相关研究推断，建议通过DOI或标题进一步验证原文。
The CYP3A43 antibody is a research tool designed to detect and study the CYP3A43 enzyme, a member of the cytochrome P450 superfamily involved in drug metabolism and steroidogenesis. CYP3A43. encoded by the *CYP3A43* gene, is a less-characterized isoform compared to its well-studied counterparts (e.g., CYP3A4. CYP3A5). It is primarily expressed in the liver, testes, and adrenal glands, suggesting roles in hormone metabolism and xenobiotic processing. However, its precise substrates, catalytic activity, and physiological relevance remain unclear due to low expression levels and overlapping functions with other CYP3A enzymes.
CYP3A43 antibodies are typically polyclonal or monoclonal antibodies developed to identify the protein in immunological assays like Western blotting (WB), immunohistochemistry (IHC), or immunofluorescence (IF). These antibodies target specific epitopes within the CYP3A43 protein, aiding in the investigation of its tissue distribution, expression patterns under varying conditions (e.g., drug exposure, disease states), and interaction with regulatory proteins. Challenges in antibody development include ensuring specificity, as CYP3A isoforms share high sequence homology. Validation often involves knockout controls or siRNA-mediated silencing.
Current research using CYP3A43 antibodies focuses on elucidating its potential role in personalized medicine, pharmacogenomics, and interindividual variability in drug responses. Despite limited studies, its unique expression profile in reproductive and endocrine tissues hints at unexplored biological functions. Commercial availability of these antibodies supports ongoing efforts to clarify CYP3A43's contributions to metabolic pathways and disease mechanisms.
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