| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CYPIVF12; F22329_1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP4F12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP4F12抗体的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Development and Application of a CYP4F12-Specific Monoclonal Antibody for Immunohistochemical Analysis"*
**作者**: Zhang L, et al.
**摘要**: 本研究开发了一种高特异性CYP4F12单克隆抗体，验证其在人肝脏和肠道组织中的表达定位，证实其在药物代谢和脂质氧化研究中的实用性。
2. **文献名称**: *"CYP4F12 Expression in Atopic Dermatitis: Correlation with Skin Barrier Dysfunction"*
**作者**: Müller R, et al.
**摘要**: 通过Western blot和免疫组化技术，利用CYP4F12抗体发现其在特应性皮炎患者表皮中表达下调，提示其与皮肤屏障功能受损相关。
3. **文献名称**: *"CYP4F12-Mediated Eicosanoid Metabolism in Colorectal Cancer Progression"*
**作者**: Tanaka K, et al.
**摘要**: 研究使用CYP4F12抗体检测结直肠癌组织，发现其表达水平与前列腺素代谢通路激活及患者预后不良显著相关。
4. **文献名称**: *"Functional Characterization of CYP4F12 in Drug Metabolism Using siRNA and Antibody-Based Inhibition"*
**作者**: Johnson B, et al.
**摘要**: 结合CYP4F12抗体与基因沉默技术，揭示该酶在肝细胞中对特定药物的氧化代谢作用，为个体化用药提供依据。
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**建议**：以上为模拟示例，实际文献请通过PubMed、Google Scholar等平台以“CYP4F12 antibody”或“CYP4F12 immunohistochemistry”为关键词检索。
The CYP4F12 antibody is a tool used to detect and study the cytochrome P450 family 4 subfamily F member 12 (CYP4F12) enzyme, which plays a role in metabolizing endogenous and exogenous compounds. CYP4F12 is part of the cytochrome P450 superfamily, known for oxidizing fatty acids, eicosanoids, and xenobiotics. This enzyme is primarily expressed in the liver, kidneys, and intestines, contributing to the metabolism of arachidonic acid derivatives, including leukotriene B4 and hydroxyeicosatetraenoic acids (HETEs), as well as certain drugs. Its genetic locus is on chromosome 19p13.12.
Research using CYP4F12 antibodies focuses on understanding its physiological roles, such as regulating inflammatory responses, lipid homeostasis, and drug clearance. These antibodies are essential in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, tissue distribution, and subcellular localization (e.g., endoplasmic reticulum or mitochondria). Dysregulation of CYP4F12 has been implicated in diseases like hypertension, cancer, and metabolic disorders, making its study clinically relevant. Monoclonal or polyclonal CYP4F12 antibodies are validated for specificity to ensure accurate detection, aiding investigations into enzyme function, genetic variants, and therapeutic targeting. Ongoing studies explore its interactions with lipid signaling pathways and potential as a biomarker or drug target.
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