| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CPJ2; CYPIIJ2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP2J2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP2J2抗体的3篇参考文献示例(注：文献信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **标题**: *"Cytochrome P450 2J2 Epoxygenase Regulates Endothelial Cell Survival and Tumor Angiogenesis"*
**作者**: Chen C, et al.
**摘要**: 研究利用CYP2J2抗体检测其在肿瘤内皮细胞中的表达，发现其通过代谢花生四烯酸生成环氧二十碳三烯酸(EETs)，促进血管生成和肿瘤生长。
2. **标题**: *"Overexpression of CYP2J2 in Human Cancers: Association with Progression and Poor Prognosis"*
**作者**: Wang H, et al.
**摘要**: 通过免疫组化(使用CYP2J2抗体)分析多种癌症组织，发现CYP2J2高表达与肿瘤转移及患者生存率降低相关，提示其作为潜在治疗靶点。
3. **标题**: *"CYP2J2-Derived Epoxyeicosatrienoic Acids in Cardioprotection"*
**作者**: Zeldin DC, et al.
**摘要**: 研究使用CYP2J2抗体确认其在心肌细胞中的定位，证明其产生的EETs通过抗炎和抗氧化机制减轻心肌缺血再灌注损伤。
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**注意**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“CYP2J2 antibody”、“CYP2J2 function”)，并参考高被引论文或权威期刊(如《Journal of Biological Chemistry》《Drug Metabolism and Disposition》)。
The CYP2J2 antibody is a specialized tool used to detect and study the cytochrome P450 2J2 (CYP2J2) enzyme, a member of the cytochrome P450 superfamily involved in xenobiotic metabolism and endogenous lipid signaling. CYP2J2 is primarily expressed in extrahepatic tissues, such as the heart, kidneys, and gastrointestinal tract, where it metabolizes arachidonic acid to produce epoxyeicosatrienoic acids (EETs). These metabolites play critical roles in cardiovascular homeostasis, including vasodilation, anti-inflammatory effects, and angiogenesis. Dysregulation of CYP2J2 has been linked to pathologies like hypertension, cancer, and diabetic complications, making it a target for therapeutic research.
The CYP2J2 antibody enables researchers to localize and quantify the enzyme in tissues or cell lines using techniques like Western blotting, immunohistochemistry, or immunofluorescence. Its specificity helps investigate CYP2J2 expression patterns under varying physiological or pathological conditions, such as tumor progression or drug interactions. Studies also utilize this antibody to explore CYP2J2's role in drug metabolism, particularly its involvement in metabolizing antihistamines and anticancer agents. As CYP2J2's EET products are increasingly recognized for their cardioprotective effects, the antibody supports research into developing enzyme-targeted therapies or biomarkers for cardiovascular and oncological diseases. Validation of antibody specificity through knockout controls or peptide blocking is essential to ensure reliable experimental outcomes.
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