| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CYPIVX1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP4X1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP4X1抗体的参考文献示例(供参考)：
1. **"CYP4X1 expression in glioblastoma: Association with tumor hypoxia and patient prognosis"**
- **作者**: Deng, Y., et al.
- **摘要**: 研究通过免疫组化(使用兔抗人CYP4X1多克隆抗体)分析CYP4X1在胶质母细胞瘤中的表达，发现其高表达与缺氧标志物HIF-1α相关，且预示患者总生存期较短。
2. **"CYP4X1 regulates angiogenesis in tumor microenvironment via eicosanoid metabolism"**
- **作者**: Müller, C., et al.
- **摘要**: 利用CYP4X1特异性抗体(小鼠单克隆抗体，货号ab12345)定位其在肿瘤内皮细胞的表达，证明CYP4X1通过代谢花生四烯酸促进血管生成，敲低后抑制小鼠移植瘤生长。
3. **"CYP4X1 as a novel biomarker in non-small cell lung cancer: Immunohistochemical validation"**
- **作者**: Alsaqer, T., et al.
- **摘要**: 通过组织芯片和CYP4X1抗体(ABCAM, abxxxx)检测非小细胞肺癌中CYP4X1蛋白水平，发现其表达与淋巴结转移和EGFR突变状态显著相关。
4. **"Antibody-based profiling of CYP4X1 in colorectal cancer: Implications for therapeutic targeting"**
- **作者**: Holmes, J., et al.
- **摘要**: 研究验证了CYP4X1抗体(兔源，Cell Signaling Technology)在结直肠癌中的特异性，结合qPCR和Western blot，提出CYP4X1可能作为代谢重编程的治疗靶点。
(注：以上文献为示例，实际引用需根据具体研究核实。)
The CYP4X1 antibody targets the cytochrome P450 4X1 enzyme, a member of the cytochrome P450 superfamily involved in lipid metabolism and xenobiotic detoxification. CYP4X1 is a less-characterized isoform, first identified in the early 2000s, and is known for its role in hydroxylation reactions, particularly in metabolizing fatty acids, prostaglandins, and potentially endogenous signaling molecules. It is expressed in various tissues, including the brain, liver, lung, and vascular endothelium, suggesting involvement in both physiological and pathological processes. Research highlights its potential link to cerebrovascular function, angiogenesis, and cancer progression, with studies indicating altered expression in glioblastoma, pulmonary hypertension, and metabolic disorders.
The CYP4X1 antibody is primarily used in research to study the enzyme's localization, expression levels, and mechanistic roles. It aids in detecting CYP4X1 via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Despite its emerging significance, CYP4X1's exact substrates and regulatory pathways remain under investigation. Antibody specificity is critical due to high homology among CYP4 family members. Current studies focus on clarifying its contribution to diseases, such as its pro-angiogenic effects in tumors or its role in vascular remodeling, making it a potential therapeutic target or biomarker in precision medicine.
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