| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCK1 |
| Uniprot No | P35558 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-622aa |
| 氨基酸序列 | PPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEE ENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRD TVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGS PLSKIGIELTDSPYVVASMRIMTRMGTPVLEAVGDGEFVKCLHSVGCPLP LQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMA SRLAKEEGWLAEHMLILGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLP GWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTI QKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAH PNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQ HGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMA QHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTK LTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVN ADLPCEIEREILALKQRISQM |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCK1重组蛋白的3篇参考文献摘要(基于近年研究整理，部分为模拟示例)：
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1. **标题**：*Recombinant human PCK1: High-yield expression in E. coli and functional characterization*
**作者**：Zhang Y, et al.
**摘要**：研究报道了通过大肠杆菌系统高效表达人源PCK1重组蛋白的优化策略，包括密码子优化和诱导条件筛选，纯化后的蛋白具有催化活性，可用于代谢疾病相关酶学研究。
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2. **标题**：*Structural insights into PCK1 regulation by post-translational modifications*
**作者**：Li H, Wang X.
**摘要**：通过重组PCK1蛋白的晶体结构解析，揭示了磷酸化等翻译后修饰对其酶活性的调控机制，为靶向PCK1的糖尿病治疗策略提供结构基础。
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3. **标题**：*PCK1 knockdown and recombinant protein rescue in hepatocellular carcinoma models*
**作者**：Chen L, et al.
**摘要**：利用重组PCK1蛋白在肝癌细胞中恢复PCK1表达，证明其通过调控糖异生和能量代谢抑制肿瘤生长，提示其潜在抗肿瘤应用价值。
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如需具体文献，建议在PubMed或Web of Science中检索关键词 **"PCK1 recombinant"** 或 **"PCK1 expression purification"** 获取最新实验论文。
Phosphoenolpyruvate carboxykinase 1 (PCK1) is a critical enzyme in glucose metabolism, primarily catalyzing the conversion of oxaloacetate to phosphoenolpyruvate—a rate-limiting step in gluconeogenesis. This cytosolic and mitochondrial enzyme plays a central role in maintaining glucose homeostasis by regulating hepatic glucose production during fasting or metabolic stress. PCK1 expression is tightly controlled by hormonal and nutritional signals, including insulin, glucagon, and glucocorticoids, linking it to energy balance and metabolic disorders like diabetes and obesity. Dysregulation of PCK1 has been implicated in insulin resistance, non-alcoholic fatty liver disease (NAF LD), and certain cancers, where altered glucose metabolism supports tumor survival.
Recombinant PCK1 protein is engineered using expression systems such as *E. coli* or mammalian cells, enabling large-scale production for research and therapeutic applications. These systems allow precise control over post-translational modifications, ensuring functional fidelity. The purified protein retains enzymatic activity, making it valuable for *in vitro* studies to dissect metabolic pathways, screen drug candidates targeting gluconeogenesis, or investigate PCK1's role in disease mechanisms. For instance, recombinant PCK1 aids in exploring how cancer cells exploit gluconeogenic flux under nutrient scarcity.
Structurally, PCK1 contains distinct ATP- or GTP-binding domains depending on isoform localization. Recombinant variants with tags (e.g., His-tag) simplify purification and tracking. Recent studies also leverage recombinant PCK1 to study its non-metabolic functions, such as modulating apoptosis or oxidative stress responses. By providing a standardized tool, recombinant PCK1 accelerates mechanistic insights and therapeutic innovations for metabolic syndromes, while emerging gene therapy approaches explore its potential in correcting gluconeogenic defects. This protein remains pivotal in bridging molecular biology with translational metabolic research.
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