| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHGC4 |
| Uniprot No | Q9Y5F7-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-692aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MQIRYPVPEE SQEGTFVGNV AQDFLLDTDS LSARRLQVAG EVNQRHFRVD LDSGALLIKN PIDREALCGL SASCIVPLEF VTEGPLEMYR AEVEIVDVND HAPRFPRQQL DLEIGEAAPP GQRFPLEKAQ DADVGSNSIS SYRLSSNEHF ALDVKKRSDG SLVPELLLEK PLDREKQSDY RLVLTAVDGG NPPRSGTAEL RVSVLDVNDN APAFQQSSYR ISVLESAPAG MVLIQLNASD PDLGPSGNVT FYFSGHTPDR VRNLFSLHPT TGKLTLLGPL DFESENYYEF DVRARDGGSP AMEQHCSLRV DLLDVNDNAP YITVTSELGT LPESAEPGTV VALISVQDPD SGSNGDVSLR IPDHLPFALK SAFRNQFSLV TAGPLDREAK SSYDIMVTAS DAGNPPLSTH RTIFLNISDV NDNPPSFFQR SHEVFVPENN RPGDLLCSLA ASDPDSGLNA LISYSLLEPR NRDVSASSFI SLNPQTGAVH ATRSFDYEQT QTLQFEVQAR DRGNPPLSST VTVRLFVLDL NDNAPAVLRP RARPGSLCPQ ALPPSVGAGH LITKVTAVDL DSGYNAWVSY QLLEAPDPSL FAVSRYAGEV RTAVPIPADL PPQKLVIVVK DSGSPPLSTS VTLLVSLEED THPVVPDLRE SSAPREGESR LTLY |
| 预测分子量 | 74 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHGC4重组蛋白的3篇示例参考文献(注：以下内容为虚构示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Protocadherin-Gamma-C4 in Neural Circuit Formation"
**作者**: Li, X., et al.
**摘要**: 本研究在大肠杆菌系统中成功表达了PCDHGC4重组蛋白，并通过体外细胞黏附实验证明其参与神经元突触特异性连接。研究发现，PCDHGC4通过钙离子依赖性机制介导细胞间相互作用，可能调控大脑皮层神经网络的发育。
2. **文献名称**: "Structural Analysis of PCDHGC4 Extracellular Domain by Cryo-EM Reveals Homophilic Binding Specificity"
**作者**: Zhang, Y., & Wang, H.
**摘要**: 利用冷冻电镜技术解析了PCDHGC4胞外结构域的三维结构，揭示了其独特的同源二聚化模式。重组蛋白的功能实验表明，其结构中的EC4-EC5结构域对结合特异性起关键作用，为原钙粘蛋白家族的功能研究提供了新视角。
3. **文献名称**: "PCDHGC4 Recombinant Protein Suppresses Tumor Metastasis via Wnt/β-Catenin Signaling Pathway"
**作者**: Chen, L., et al.
**摘要**: 通过哺乳动物细胞表达系统获得高纯度PCDHGC4重组蛋白，并发现其在体外可抑制肺癌细胞迁移。机制研究表明，PCDHGC4通过竞争性结合β-catenin调控Wnt通路活性，提示其作为肿瘤治疗靶点的潜力。
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**建议**：实际研究中，可通过PubMed、Web of Science或Google Scholar检索关键词“PCDHGC4 recombinant protein”或“PCDHGC4 expression”获取最新文献。若研究较少，可扩展至原钙粘蛋白家族(protocadherin)的共性研究。
**Background of PCDHGC4 Recombinant Protein**
Protocadherin gamma subfamily C4 (PCDHGC4) is a member of the protocadherin gamma gene cluster, part of the cadherin superfamily of calcium-dependent cell adhesion molecules. Protocadherins are predominantly expressed in the nervous system and play critical roles in neural circuit formation, synaptic specificity, and neuronal survival. The gamma protocadherins, including PCDHGC4. are characterized by their extracellular cadherin repeats, a transmembrane domain, and a variable cytoplasmic tail that mediates intracellular signaling.
PCDHGC4 is implicated in cell-cell recognition and adhesion, contributing to the establishment of complex neural networks during development. Its expression is tightly regulated, with studies suggesting roles in axon guidance, dendritic arborization, and the prevention of neuronal apoptosis. Dysregulation of protocadherins has been linked to neurodevelopmental disorders, psychiatric conditions, and cancers, highlighting their biological and clinical relevance.
Recombinant PCDHGC4 protein is engineered to enable functional studies *in vitro* or *in vivo*. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), the recombinant form retains key structural and functional domains, allowing researchers to investigate its binding partners, signaling pathways, and role in cellular interactions. Purification tags (e.g., His-tag) are often incorporated to facilitate isolation.
Current research leverages PCDHGC4 recombinant protein to explore its therapeutic potential, such as in neural repair or cancer immunotherapy, and to develop diagnostic tools targeting protocadherin-related pathologies. Its study also advances understanding of how cell-surface diversity drives neural connectivity and tissue organization.
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