首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨成型蛋白4(BMP-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BMP4含量。BMP4，全称骨形态发生蛋白4，是一种多效性配体蛋白，属于TGFβ家族，参与细胞增殖、分化和凋亡的调节。其研究机制涉及多种信号通路，如Notch、Wnt/β-catenin等，对细胞命运决定和胚胎发育有重要作用。试剂盒检测范围为0.625 pg/mL-40 pg/mL，为科研人员探索BMP - 4在骨骼疾病模型构建、干细胞定向分化实验、组织修复机制研究及药物干预效果评估提供可靠工具；支持基础研究中不同实验条件的检测需求，例如体外细胞培养上清分析、动物模型组织匀浆检测或循环系统中蛋白动态监测等本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bmp4 ELISA kit; Bmp-4 ELISA kit; Dvr-4Bone morphogenetic protein 4 ELISA kit; BMP-4 ELISA kit; Bone morphogenetic protein 2B ELISA kit; BMP-2B ELISA kit
缩写：BMP4
Uniprot No.：P21275
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-112 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-110 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 402.6572.6772.6672.509 202.2122.3022.2572.099 101.5831.6831.6331.475 51.0421.0821.0620.904 2.50.5970.6220.6100.452 1.250.3840.4010.3930.235 0.6250.2880.3020.2950.137 00.1620.1540.158 | ||||||
货期：3-5 working days
×
