| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FAM31A; KIAA1608 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DENND1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与DENND1A抗体相关的代表性文献摘要概括：
1. **文献名称**：*DENND1A Variants in Polycystic Ovary Syndrome*
**作者**：McAllister et al.
**摘要**：研究使用DENND1A特异性抗体通过Western blot和免疫组化分析，发现DENND1A在卵巢颗粒细胞中的异常表达与多囊卵巢综合征(PCOS)相关，揭示了其作为PCOS潜在生物标志物的作用。
2. **文献名称**：*Regulation of DENND1A by Hormonal Signaling Pathways*
**作者**：Teixeira et al.
**摘要**：通过DENND1A抗体检测，发现雄激素和胰岛素信号通路调控DENND1A的剪接变体表达，阐明了其在代谢和生殖疾病中的分子机制。
3. **文献名称**：*DENND1A in Cellular Vesicle Trafficking*
**作者**：Dumont et al.
**摘要**：利用DENND1A抗体进行免疫沉淀和共聚焦显微镜观察，证实DENND1A通过调控Rab35-GTPase活性参与细胞内膜运输过程，影响突触形成和胞吐作用。
4. **文献名称**：*DENND1A Expression in Neural Tissues*
**作者**：Ohara et al.
**摘要**：研究采用DENND1A抗体进行组织特异性分析，发现其在哺乳动物脑部神经元中高表达，提示其可能参与神经发育相关信号传导通路。
注：以上文献信息为示例性概括，实际研究需通过PubMed或Google Scholar以“DENND1A antibody”为关键词检索具体文献。
The DENND1A antibody is a crucial tool for studying the DENND1A protein, a member of the Differentially Expressed in Normal and Neoplastic Cells (DENN) domain-containing protein family. DENND1A functions as a guanine nucleotide exchange factor (GEF) for Rab35. a small GTPase involved in membrane trafficking, cytoskeletal dynamics, and cytokinesis. It exists in two major splice variants: DENND1A.V1 (long isoform) and DENND1A.V2 (short isoform), which exhibit distinct cellular localization and regulatory roles. Research has linked DENND1A to several physiological and pathological processes, including endocrine regulation, neurotransmitter release, and cancer progression. Notably, genetic studies associate DENND1A polymorphisms with polycystic ovary syndrome (PCOS), making its study clinically relevant.
DENND1A antibodies are typically produced in rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable detection of DENND1A isoforms (≈110 kDa for V1 and ≈55 kDa for V2) through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity. Commercially available antibodies target various epitopes, with some distinguishing between isoforms. Researchers use these antibodies to investigate DENND1A's role in Rab35 activation, endosomal trafficking, and its potential as a therapeutic target in metabolic disorders or cancers. Proper antibody selection remains critical due to potential cross-reactivity with other DENN family proteins.
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