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产品描述：人骨成型蛋白7(BMP-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的BMP7含量。BMP7（骨形态发生蛋白7）是一种多效性配体蛋白，属于TGF-β家族，参与调节癌细胞增殖、侵袭和迁移，与多种人类肿瘤相关。其通过结合ALK2或ACVR2A/BMPR2受体，激活信号传导，调控细胞生长和分化。研究显示，BMP7在肿瘤发生发展中具有双重作用，既可抑制也可促进肿瘤发展。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验中BMP-7表达水平的科研分析，例如探究骨骼发育机制、组织再生调控、疾病模型中BMP-7信号通路的变化，或评估药物/基因干预对BMP-7分泌的影响。兼容多种生物样本，可满足细胞实验、动物模型或临床前研究中BMP-7动态监测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP 7 ELISA Kit; BMP-7 ELISA Kit; Bmp7 ELISA Kit; BMP7_HUMAN ELISA Kit; Bone morphogenetic protein 7 ELISA Kit; Bone morphogenic protein 7 ELISA Kit; Eptotermin alfa ELISA Kit; OP 1 ELISA Kit; OP-1 ELISA Kit; OP1 ELISA Kit; Osteogenic protein 1 ELISA Kit
缩写：BMP7
Uniprot No.：P18075
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5482.6872.6182.4495001.9821.8241.9031.7342501.1381.1671.1530.9841250.6530.6680.6610.49262.50.4050.4120.4090.24031.20.2870.2740.2810.11215.60.2200.2260.2230.05400.1680.1690.169 | ||||||
货期：3-5 working days
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