| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是模拟生成的关于DEF8抗体的参考文献示例(注：实际文献可能需要通过学术数据库验证，以下内容仅供格式参考)：
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1. **标题**：*DEF8 regulates innate immune signaling through interaction with STING*
**作者**：Smith A, et al.
**摘要**：本研究揭示了DEF8蛋白通过与STING蛋白相互作用调控I型干扰素通路的机制。作者利用DEF8特异性抗体进行免疫共沉淀实验，发现DEF8缺失会抑制病毒诱导的干扰素产生，提示其在抗病毒免疫中的关键作用。
2. **标题**：*Development of a monoclonal antibody targeting DEF8 for autophagy studies*
**作者**：Zhang L, et al.
**摘要**：研究团队开发了一种高特异性抗DEF8单克隆抗体，并通过免疫荧光和Western blot验证其在细胞自噬模型中的应用。结果显示DEF8在自噬体形成过程中与LC3蛋白共定位，提示其参与自噬调控。
3. **标题**：*DEF8 expression profiling in T-cell lymphoma using immunohistochemistry*
**作者**：Kim H, et al.
**摘要**：通过DEF8抗体进行组织免疫组化分析，发现DEF8在侵袭性T细胞淋巴瘤中显著高表达，且与患者预后不良相关，表明其可作为潜在肿瘤标志物。
4. **标题**：*Structural characterization of DEF8 and antibody epitope mapping*
**作者**：Garcia R, et al.
**摘要**：研究解析了DEF8蛋白的C端结构域，并利用多克隆抗体鉴定其功能性表位，为理解DEF8在细胞信号转导中的作用提供了结构生物学依据。
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如需查找真实文献，建议在PubMed或Google Scholar中检索关键词“DEF8 antibody”、“DEF8 immune function”或结合具体研究领域(如“DEF8 cancer”)。请注意，DEF8相关研究目前可能较为有限，部分文献可能聚焦于其基因功能而非抗体应用。
DEF8 (Differentially Expressed in FDCP 8), also known as MAPK8IP3 or JIP3. is a scaffolding protein involved in regulating intracellular signaling pathways, particularly the c-Jun N-terminal kinase (JNK) and mitogen-activated protein kinase (MAPK) cascades. It was initially identified through its differential expression in hematopoietic cells. DEF8 contains multiple protein interaction domains, including a SH3-binding motif and coiled-coil regions, enabling it to assemble signaling complexes that modulate stress responses, apoptosis, and neuronal development.
Antibodies targeting DEF8 are essential tools for studying its role in vesicular transport, autophagy, and lysosomal positioning, as DEF8 interacts with components like kinesin motors and adaptor proteins. Research links DEF8 dysfunction to neurodegenerative disorders (e.g., ALS), cancer metastasis, and immune regulation anomalies. DEF8 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to assess protein expression, localization, and molecular interactions. Commercial DEF8 antibodies are typically validated for specificity against human, mouse, or rat homologs, aiding mechanistic studies across disease models. Despite its diverse roles, DEF8's regulatory mechanisms remain under investigation, emphasizing the continued need for reliable antibodies in ongoing research.
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