| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DBX; DDX3; HLP2; DDX14; CAP-Rf; MRX102 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DDX3X |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDX3X抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*DDX3X interacts with the WNT/β-catenin signaling pathway to regulate cancer cell proliferation*
**作者**：Lai MC, Lee YH, Tarn WY
**摘要**：本研究通过免疫共沉淀(Co-IP)和Western blot技术，使用DDX3X特异性抗体验证了DDX3X与WNT信号通路中β-catenin的相互作用，揭示了其在肝癌细胞增殖中的调控机制。抗体被用于检测DDX3X的蛋白质表达水平及亚细胞定位。
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2. **文献名称**：*Germline mutations in DDX3X are a common cause of intellectual disability in females*
**作者**：Snijders Blok L, Madsen E, Juusola J, et al.
**摘要**：该研究通过免疫组化(IHC)和Western blot分析，利用DDX3X抗体检测患者脑组织及细胞模型中DDX3X的蛋白表达异常，发现DDX3X基因突变导致女性智力障碍的分子机制，强调了抗体在突变体功能验证中的关键作用。
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3. **文献名称**：*Targeting DDX3X as a therapeutic approach for HIV-1 infection*
**作者**：Yedavalli VS, Neuveut C, Chi YH, et al.
**摘要**：研究通过RNA干扰和DDX3X抗体介导的功能抑制实验，证明DDX3X在HIV-1病毒复制中不可或缺。抗体被用于验证DDX3X与病毒蛋白的相互作用及药物干预后的表达变化，为抗病毒治疗提供了新靶点。
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这些文献展示了DDX3X抗体在癌症机制、神经发育疾病及病毒研究中的广泛应用，涵盖蛋白质互作、突变分析和治疗靶点验证等领域。如需具体实验细节，建议查阅原文。
The DDX3X antibody is a crucial tool in studying the DDX3X protein, encoded by the *DDX3X* gene located on the X chromosome. DDX3X belongs to the DEAD-box RNA helicase family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs, and plays essential roles in RNA metabolism, including splicing, nucleocytoplasmic transport, translation, and stress granule formation. It is implicated in diverse cellular processes, such as cell cycle regulation, innate immunity, and viral replication. Mutations or dysregulation of DDX3X are linked to multiple pathologies, including cancers (e.g., medulloblastoma, leukemia), neurodevelopmental disorders (e.g., intellectual disability, autism), and viral infections (e.g., HIV, hepatitis C).
DDX3X antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help elucidate DDX3X's functional mechanisms in disease contexts, such as its oncogenic or tumor-suppressive roles in cancer, or its interplay with viral proteins. Recent studies also explore DDX3X as a potential therapeutic target, driving demand for high-specificity antibodies. Commercial DDX3X antibodies are typically validated for cross-reactivity and batch consistency, though variations exist between monoclonal and polyclonal versions. Their application spans basic research, biomarker discovery, and preclinical drug development, underscoring their importance in molecular and translational studies.
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