| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MCSF |
| Entrez GeneID | 1435 |
| clone | 2D10 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CSF1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDX23抗体的3篇参考文献示例(注：文献为虚构，仅作格式参考)：
1. **文献名称**：*DDX23 interacts with HCV NS5B and modulates viral RNA replication*
**作者**：Li, X., et al.
**摘要**：本研究通过免疫共沉淀(Co-IP)和Western blot技术，证实DDX23与丙肝病毒(HCV)NS5B蛋白相互作用，促进病毒RNA复制。实验中采用小鼠来源的DDX23单克隆抗体(货号ab12345.Abcam)，验证了DDX23在宿主细胞中的定位及其在病毒复制中的功能。
2. **文献名称**：*Overexpression of DDX23 in breast cancer correlates with poor prognosis*
**作者**：Wang, Y., et al.
**摘要**：通过免疫组化(IHC)分析乳腺癌组织样本，发现DDX23在肿瘤组织中高表达，且与患者生存率呈负相关。研究使用兔多克隆DDX23抗体(Proteintech, 1:500稀释)，证实DDX23可能通过调控RNA剪接促进肿瘤侵袭。
3. **文献名称**：*DDX23 regulates neuronal RNA splicing in Alzheimer’s disease models*
**作者**：Smith, J., et al.
**摘要**：在阿尔茨海默病模型中，DDX23通过调控tau蛋白前体mRNA的剪接参与病理进程。研究利用DDX23抗体(Santa Cruz, sc-555)进行Western blot和免疫荧光，发现DDX23在神经元中的异常聚集与疾病进展相关。
**注**：以上文献为示例，实际引用需根据真实研究调整。建议通过PubMed或Google Scholar以“DDX23 antibody”或“DDX23 + [研究领域]”为关键词检索最新文献。
The DDX23 antibody is a crucial tool for studying the DEAD-box RNA helicase DDX23. also known as PRP28. DDX23 belongs to the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif, and plays a pivotal role in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, and viral RNA processing. It functions as an ATP-dependent RNA helicase, facilitating structural rearrangements during spliceosome assembly by unwinding RNA duplexes. DDX23 is essential for the catalytic activation of the spliceosome, particularly during the transition from the U1/U2 snRNP-containing complex to the U4/U6.U5 tri-snRNP.
Research links DDX23 to various diseases. It is implicated in cancer progression, with studies showing overexpression in certain malignancies, correlating with poor prognosis. Additionally, DDX23 interacts with viral proteins, such as those from hepatitis C virus (HCV) and herpes simplex virus 1 (HSV-1), suggesting a role in viral replication and host immune evasion.
The DDX23 antibody enables detection and quantification of DDX23 in cellular and tissue samples via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It is widely used to investigate DDX23's functional mechanisms, subcellular localization, and interactions with RNA or partner proteins. Validated antibodies are critical for ensuring specificity, given the structural similarities among DEAD-box proteins. Recent studies also utilize DDX23 antibodies to explore its potential as a therapeutic target in oncology and virology.
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