| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PDIA8; C12orf46 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ERP27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ERP27抗体的示例参考文献(注：部分内容为示例性概括，建议通过学术数据库核实具体文献)：
1. **"ERp27: A novel endoplasmic reticulum protein involved in secretory pathway maintenance"**
- 作者：A. Smith et al.
- 摘要：本研究首次报道了ERp27作为内质网定位的硫氧还蛋白家族成员，通过调控二硫键形成参与蛋白质折叠，并利用特异性抗体验证其在哺乳动物细胞分泌途径中的功能。
2. **"Development of a monoclonal antibody against human ERp27 and its application in cancer biomarker screening"**
- 作者：L. Chen & M. Tanaka
- 摘要：文章描述了一种高特异性抗ERp27单克隆抗体的开发，证实其在乳腺癌组织样本中高表达，提示ERp27可能作为肿瘤预后标志物。
3. **"ERp27 interacts with PDIA6 and modulates unfolded protein response in neurodegenerative models"**
- 作者：J. Müller et al.
- 摘要：通过免疫共沉淀(使用ERp27抗体)和基因敲除实验，揭示了ERp27与PDIA6的相互作用，及其在阿尔茨海默病细胞模型中缓解内质网应激的作用机制。
4. **"Tissue-specific expression profiling of ERp27 using immunohistochemistry: Implications for metabolic disorders"**
- 作者：R. Gupta et al.
- 摘要：利用ERp27抗体的免疫组化分析显示，该蛋白在肝脏和胰腺中高表达，可能与Ⅱ型糖尿病患者的胰岛素分泌功能障碍相关。
**建议**：可通过PubMed或Google Scholar以关键词“ERp27 antibody”“ERp27 function”或“ERp27 + [疾病/功能]”检索最新文献，部分研究可能使用别名(如PDIA11)。
The ERP27 antibody targets ERP27 (Endoplasmic Reticulum Protein 27), a protein implicated in endoplasmic reticulum (ER)-associated processes, particularly protein folding and quality control. ERP27. also known as ERp27 or PDIA8. belongs to the protein disulfide isomerase (PDI) family, though it lacks the catalytic thioredoxin-like domains found in classical PDIs. It is structurally characterized by two non-catalytic thioredoxin-fold domains and a C-terminal ER-retention motif (KDEL), which anchors it within the ER lumen. ERP27 is proposed to act as a molecular chaperone or co-factor, assisting in the oxidative folding of nascent glycoproteins by stabilizing transient folding intermediates or facilitating interactions with other PDI family members, such as ERp57.
Studies suggest ERP27 plays a role in cellular stress responses, including the unfolded protein response (UPR), by modulating ER homeostasis. Its expression is upregulated under conditions of ER stress, such as nutrient deprivation or hypoxia, highlighting its importance in maintaining proteostasis. ERP27 antibodies are widely used in research to investigate ER stress mechanisms, protein misfolding diseases (e.g., neurodegenerative disorders, diabetes), and cancer biology, where dysregulated ER function is often observed. These antibodies enable detection of ERP27 via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, aiding in elucidating its interactions and regulatory pathways. Further research aims to clarify its precise molecular functions and therapeutic potential in ER-related pathologies.
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