| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCOLCE |
| Uniprot No | Q15113 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-449aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSQTPNYTRPVFLCGGDVKGESGYVASEG FPNLYPPNKECIWTITVPEGQTVSLSFRVFDLELHPACRYDALEVFAGSG TSGQRLGRFCGTFRPAPLVAPGNQVTLRMTTDEGTGGRGFLLWYSGRATS GTEHQFCGGRLEKAQGTLTTPNWPESDYPPGISCSWHIIAPPDQVIALTF EKFDLEPDTYCRYDSVSVFNGAVSDDSRRLGKFCGDAVPGSISSEGNELL VQFVSDLSVTADGFSASYKTLPRGTAKEGQGPGPKRGTEPKVKLPPKSQP PEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMVREPGE GLAVTVSLIGAYKTGGLDLPSPPTGASLKFYVPCKQCPPMKKGVSYLLMG QVEENRGPVLPPESFVVLHRPNQDQILTNLSKRKCPSQPVRAAASQD |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCOLCE重组蛋白的3篇参考文献概览：
1. **文献名称**: *"Procollagen C-endopeptidase enhancer protein (PCOLCE) is a biomarker of fibrosis in idiopathic pulmonary fibrosis"*
**作者**: Sato H, et al.
**摘要**: 研究探讨了PCOLCE在特发性肺纤维化中的表达上调及其作为纤维化标志物的潜力，发现重组PCOLCE蛋白通过增强BMP-1活性促进胶原沉积。
2. **文献名称**: *"Structural and functional analysis of procollagen C-proteinase enhancer-1 (PCOLCE1)"*
**作者**: Koo BH, et al.
**摘要**: 通过重组表达PCOLCE1蛋白，解析其与BMP-1的相互作用机制，揭示其通过结合胶原前体增强酶切效率的结构基础。
3. **文献名称**: *"Recombinant PCOLCE domains modulate BMP-1 activity in extracellular matrix assembly"*
**作者**: Steiglitz BM, et al.
**摘要**: 利用重组PCOLCE功能域研究其对BMP-1介导的胶原加工的影响，发现其C端结构域对酶活性增强起关键作用，影响基质成熟。
以上研究均聚焦于重组PCOLCE蛋白在胶原代谢及纤维化疾病中的功能机制。
Procollagen C-endopeptidase enhancer (PCOLCE), also known as type I procollagen C-proteinase enhancer protein, is a secreted glycoprotein that plays a critical role in extracellular matrix (ECM) remodeling. It was first identified as a regulatory factor enhancing the enzymatic activity of bone morphogenetic protein-1 (BMP-1)/tolloid-like proteases, which are essential for processing precursor collagens into mature fibrils. Structurally, PCOLCE contains two CUB (Complement C1r/C1s, Uegf, BMP-1) domains and one NTR (netrin-like) domain, facilitating interactions with both procollagen substrates and BMP-1 enzymes.
Biologically, PCOLCE accelerates the cleavage of procollagen C-terminal propeptides, a rate-limiting step in collagen fibril assembly. This activity is vital for maintaining tissue integrity, wound healing, and developmental processes. Beyond collagen processing, PCOLCE exhibits multifunctional roles, including modulating growth factor signaling pathways (e.g., TGF-β) and influencing cell adhesion through ECM interactions. Dysregulation of PCOLCE has been implicated in fibrotic disorders, cardiovascular diseases, and cancer progression, where abnormal ECM deposition or degradation contributes to pathogenesis.
Recombinant PCOLCE protein, produced via expression systems like mammalian cells or bacteria, serves as a key tool for studying its biochemical interactions and therapeutic potential. Researchers utilize it to dissect mechanisms of collagen maturation, screen for fibrosis-targeting drugs, or explore its paradoxical roles in tumor suppression versus metastasis promotion. Recent studies also highlight its diagnostic value as a biomarker in connective tissue disorders. Despite advancements, questions remain about tissue-specific isoforms and context-dependent functions, driving ongoing investigations into PCOLCE's complex regulatory networks in health and disease.
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