| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ACTC; ASD5; CMD1R; CMH11; LVNC4 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ACTC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ACTC1抗体的代表性文献(虚构示例，仅作格式参考)：
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1. **标题**: *ACTC1 expression in hypertrophic cardiomyopathy: an immunohistochemical study*
**作者**: Smith A, et al.
**摘要**: 本研究使用ACTC1单克隆抗体(克隆号HHF35)，通过免疫组化分析肥厚型心肌病患者心肌组织，发现ACTC1蛋白表达水平与心肌纤维化程度呈正相关，提示其在疾病进展中的作用。
2. **标题**: *Development of a novel polyclonal antibody for cardiac α-actin detection*
**作者**: Lee B, et al.
**摘要**: 报道了一种新型兔源多克隆抗体的制备与验证，该抗体针对人ACTC1蛋白C端表位，经Western blot和免疫荧光验证具有高特异性，适用于胚胎心脏发育研究。
3. **标题**: *Comparative analysis of ACTC1 antibody specificity across species*
**作者**: Garcia C, et al.
**摘要**: 系统性比较了6种商用ACTC1抗体在小鼠、斑马鱼和人类样本中的交叉反应性，发现克隆号A4.951在哺乳动物中特异性最佳，但对鱼类样本存在假阳性。
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注：以上文献信息为模拟生成，实际引用需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar检索真实研究。建议使用关键词 **"ACTC1 antibody validation"** 或 **"ACTC1 immunohistochemistry"** 进行精准查找。
The ACTC1 antibody is a research tool targeting the alpha-cardiac actin protein, encoded by the ACTC1 gene. This gene is part of the actin family, which comprises essential cytoskeletal proteins involved in cell structure, motility, and muscle contraction. ACTC1 is predominantly expressed in cardiac muscle, where it plays a critical role in sarcomere organization and contractile function. It constitutes a major component of the thin filaments in cardiomyocytes, interacting with myosin to enable heart muscle contraction.
ACTC1 antibodies are widely used in cardiovascular research to study protein expression, localization, and regulatory mechanisms in both healthy and diseased tissues. They are applied in techniques such as immunohistochemistry, Western blotting, and immunofluorescence to investigate conditions like hypertrophic cardiomyopathy, dilated cardiomyopathy, and congenital heart defects. Mutations in ACTC1 have been linked to these cardiac disorders, making the antibody valuable for elucidating disease pathways.
Additionally, ACTC1 antibodies occasionally aid in studying skeletal muscle biology, as low levels of alpha-cardiac actin may be present in skeletal muscle. Their specificity is rigorously validated to avoid cross-reactivity with other actin isoforms (e.g., alpha-skeletal or cytoplasmic actins). As cardiac diseases remain a leading cause of mortality, ACTC1 antibodies serve as key tools for advancing diagnostic and therapeutic strategies targeting actin-related pathologies.
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