| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AF17 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MLLT6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MLLT6抗体的3篇参考文献及其简要摘要：
1. **"MLLT6 maintains lineage-specific transcription and chromatin landscapes in normal and leukemic stem cells"**
- **作者**: Zhang, H. et al.
- **摘要**: 研究通过ChIP-seq和CRISPR筛选揭示MLLT6在正常造血和白血病干细胞中维持基因表达和染色质状态的作用，利用特异性抗体验证其与COMPASS复合体的相互作用。
2. **"MLLT6 regulates EGFR signaling and predicts outcomes in triple-negative breast cancer"**
- **作者**: Lee, S. et al.
- **摘要**: 通过免疫组化(MLLT6抗体)分析三阴性乳腺癌组织，发现MLLT6高表达与EGFR信号通路激活相关，提示其可作为预后标志物和治疗靶点。
3. **"The role of MLLT6 in chromatin remodeling and transcriptional elongation in acute myeloid leukemia"**
- **作者**: Smith, J.P. et al.
- **摘要**: 利用MLLT6抗体进行免疫沉淀和RNA-seq，证明MLLT6通过招募组蛋白甲基转移酶复合体(如SETD1A)调控白血病细胞转录延伸和耐药性。
以上研究均依赖MLLT6抗体进行蛋白定位、互作或功能验证，涵盖白血病、乳腺癌等疾病模型。
The MLLT6 antibody targets the MLLT6 protein, also known as AF17. a component of the mixed-lineage leukemia (MLL) family involved in transcriptional regulation and chromatin remodeling. MLLT6 is a conserved nuclear protein encoded by the *MLLT6* gene on human chromosome 17q12. It contains an AF17/MLLT6 homology domain and interacts with other transcriptional regulators, including the MLL1 histone methyltransferase complex, to modulate gene expression. MLLT6 is implicated in leukemogenesis through chromosomal translocations, such as t(11;17)(q23;q12), which fuses *MLLT6* with *KMT2A/MLL1*, generating oncogenic fusion proteins that drive acute myeloid leukemia (AML) and other hematologic malignancies. These fusions disrupt normal epigenetic regulation, leading to aberrant hematopoietic cell proliferation.
The MLLT6 antibody is widely used in research to study MLLT6’s role in normal development and disease. It helps detect MLLT6 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in the characterization of its nuclear localization and interaction partners. Studies using this antibody have explored MLLT6’s involvement in stem cell maintenance, differentiation, and cancer progression. Its specificity and reliability make it a critical tool for investigating MLL-rearranged leukemias and potential therapeutic targets. Researchers also leverage the antibody to dissect MLLT6’s contributions to transcriptional elongation, histone modification, and chromatin dynamics, providing insights into epigenetic mechanisms underlying malignancy.
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