| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SAMM50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SAMM50抗体的相关文献摘要概览：
1. **文献名称**: *SAMM50 regulates mitochondrial lipid metabolism and liver homeostasis*
**作者**: Li, X., et al.
**摘要**: 该研究通过免疫共沉淀和Western blot技术，发现SAMM50蛋白在线粒体脂质代谢中与特定脂质转运蛋白相互作用。使用SAMM50抗体证实其在非酒精性脂肪肝病(NAFLD)患者肝组织中表达下调，提示其功能缺失可能导致线粒体功能障碍和脂质堆积。
2. **文献名称**: *Mitochondrial SAMM50 modulates PINK1/Parkin-mediated mitophagy*
**作者**: Zhang, Y., et al.
**摘要**: 研究利用SAMM50抗体进行免疫荧光定位，发现SAMM50通过调控线粒体膜动态平衡参与PINK1/Parkin介导的线粒体自噬。实验表明，敲低SAMM50会抑制自噬小体形成，导致神经细胞中线粒体异常聚集。
3. **文献名称**: *Genetic variation in SAMM50 is associated with NAFLD severity*
**作者**: Kim, S., et al.
**摘要**: 通过全基因组关联分析，发现SAMM50基因多态性与NAFLD进展相关。免疫组化实验显示，携带风险等位基因的患者肝细胞内SAMM50蛋白水平显著降低，提示其抗体可作为评估线粒体功能障碍的生物标志物。
4. **文献名称**: *Structural insights into SAMM50-containing mitochondrial complexes*
**作者**: Wang, L., et al.
**摘要**: 采用冷冻电镜和SAMM50抗体标记技术解析了SAMM50与MTCH2、MTFP1组成的线粒体膜复合物结构，揭示了其在维持线粒体嵴形态和呼吸链组装中的关键作用，为相关代谢疾病机制提供结构基础。
注：以上内容为模拟文献摘要，实际引用请以真实发表的论文为准。建议通过PubMed或Google Scholar以“SAMM50 antibody”、“SAMM50 function”等关键词检索最新研究。
The SAMM50 antibody targets the SAMM50 protein, a key component of the mitochondrial sorting and assembly machinery (SAM) complex. SAMM50. also known as sorting and assembly machinery component 50. plays a critical role in the biogenesis and maintenance of mitochondrial membrane structure by facilitating the assembly of β-barrel proteins into the outer mitochondrial membrane. It interacts with other SAM complex components, such as SAMM35 and MTCH2. to ensure proper protein import and membrane topology. The SAMM50 protein contains a central SAM domain and a N-terminal PB1 domain, which mediate protein-protein interactions essential for its function.
Antibodies against SAMM50 are widely used in research to study mitochondrial dynamics, protein import mechanisms, and mitochondrial-associated pathologies, including neurodegenerative diseases, metabolic disorders, and cancer. They enable detection of SAMM50 expression levels via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in the exploration of mitochondrial dysfunction in disease models. Additionally, SAMM50 antibodies help elucidate its role in mitochondrial quality control pathways, such as mitophagy, and its interaction with regulatory proteins like PHB2. These tools are vital for advancing understanding of mitochondrial biology and developing therapeutic strategies targeting mitochondrial disorders.
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