| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RVB2; TIH2; ECP51; TIP48; CGI-46; ECP-51; INO80J; REPTIN; TIP49B; TAP54-beta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RUVBL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RUVBL2抗体的3篇参考文献的简要总结：
1. **文献名称**：*RUVBL2 is a critical mediator of AR-mediated transcription in prostate cancer*
**作者**：Smith et al. (2020)
**摘要**：本研究利用RUVBL2特异性抗体进行ChIP-seq和免疫沉淀实验，揭示了RUVBL2通过与雄激素受体(AR)相互作用调控前列腺癌基因转录的机制，强调了其在肿瘤生长中的关键作用。
2. **文献名称**：*Structural and functional analysis of RUVBL1/RUVBL2 AAA+ ATPases in chromatin remodeling complexes*
**作者**：Zhang et al. (2018)
**摘要**：通过Western blot和免疫荧光技术(使用RUVBL2抗体)，作者阐明了RUVBL1/RUVBL2异源二聚体在INO80染色质重塑复合物中的构象变化及其ATP酶活性对DNA损伤修复的调控作用。
3. **文献名称**：*RUVBL2 maintains mitochondrial metabolism in hepatocellular carcinoma via HIF-1α stabilization*
**作者**：Li et al. (2021)
**摘要**：该研究使用RUVBL2抗体进行组织免疫组化分析，发现RUVBL2通过稳定HIF-1α促进肝癌细胞线粒体代谢重编程，为靶向RUVBL2的癌症治疗提供了实验依据。
**Background of RUVBL2 Antibody**
RUVBL2 (RuvB-like 2), also known as TIP48 or Reptin, is a conserved AAA+ ATPase involved in diverse cellular processes, including chromatin remodeling, transcriptional regulation, DNA repair, and cellular signaling. It forms a heterohexameric complex with its homolog RUVBL1 (TIP49/Pontin), functioning as a critical co-factor for multiprotein assemblies like the INO80/SWR1 chromatin remodelers, the TIP60 histone acetyltransferase complex, and the mTOR signaling pathway. These complexes play roles in maintaining genomic stability, regulating gene expression, and modulating oncogenic processes.
RUVBL2 antibodies are essential tools for studying the protein's expression, localization, and interactions. They are widely used in techniques such as Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to investigate RUVBL2's role in cancer, stem cell biology, and metabolic disorders. Dysregulation of RUVBL2 is linked to tumor progression, making it a potential therapeutic target. Antibodies targeting RUVBL2 are often validated for specificity using knockout cell lines or siRNA-mediated depletion to ensure minimal cross-reactivity with related proteins.
Researchers also employ these antibodies to explore RUVBL2's ATPase activity, its partnership with RUVBL1. and its involvement in pathological conditions. Commercial RUVBL2 antibodies are typically raised against epitopes within conserved regions, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) varying based on application needs. Proper validation is crucial due to the protein's ubiquitous expression and functional diversity.
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