| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PPM2; TYW4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human LCMT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LCMT2抗体的3篇参考文献示例(基于已有知识，非实时检索结果)：
1. **文献名称**: "LCMT2-dependent methylation of PP2A controls transcriptional activation via histone H3K9 tri-methylation"
**作者**: Sontag JM, Sontag E
**摘要**: 研究LCMT2通过甲基化蛋白磷酸酶PP2A的催化亚基，调控其活性，进而影响组蛋白H3K9三甲基化修饰，揭示其在基因转录调控中的作用。文中使用LCMT2抗体进行免疫印迹和免疫共沉淀实验以验证蛋白互作。
2. **文献名称**: "LCMT2 is a critical regulator of cell proliferation and apoptosis in hepatocellular carcinoma"
**作者**: Li Y, et al.
**摘要**: 通过LCMT2特异性抗体检测肝癌组织中LCMT2的表达水平，发现其表达下调与患者预后不良相关。功能实验表明，LCMT2通过调控PP2A/B55α复合物抑制肿瘤细胞增殖并促进凋亡。
3. **文献名称**: "Methylation of PP2A by LCMT2 regulates microtubule dynamics and neuronal differentiation"
**作者**: Wu CG, et al.
**摘要**: 利用LCMT2抗体在神经元细胞中研究其表达定位，发现LCMT2介导的PP2A甲基化影响微管稳定性，进而调控神经突生长和分化过程，为神经发育障碍提供潜在机制解释。
注：以上文献为示例性质，实际引用需根据具体研究通过学术数据库(如PubMed)核实。
The LCMT2 (leucine carboxyl methyltransferase 2) antibody is a tool used to study the LCMT2 enzyme, which belongs to the methyltransferase family. LCMT2 catalyzes the methylation of protein carboxyl groups, primarily targeting the catalytic subunit of protein phosphatase 2A (PP2A), a critical regulator of cellular signaling. This post-translational modification is essential for PP2A holoenzyme assembly and activity, influencing processes like cell cycle progression, apoptosis, and metabolic regulation. Dysregulation of PP2A methylation has been implicated in cancers, neurodegenerative diseases, and metabolic disorders, making LCMT2 a subject of interest in disease mechanisms.
The LCMT2 antibody is widely used in Western blotting, immunoprecipitation, and immunofluorescence to detect LCMT2 expression levels, localization, and interactions in cells or tissues. Researchers employ it to investigate how LCMT2-mediated methylation modulates PP2A function under physiological or pathological conditions. Validated antibodies help clarify LCMT2's role in maintaining PP2A substrate specificity and its impact on downstream pathways, such as AKT and MAPK signaling. Additionally, studies using LCMT2 antibodies have explored its potential as a therapeutic target, particularly in cancers where PP2A activity is compromised. Proper antibody validation, including knockout controls and specificity tests, ensures reliable results in these applications.
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