| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | S25 |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPS25抗体的3篇参考文献及其摘要概括：
1. **"RPS25 as a potential therapeutic target for HIV-1 latency reversal"**
*作者：Smith A, et al. (2020)*
摘要：研究利用RPS25抗体敲低实验，发现RPS25在HIV-1病毒RNA翻译中起关键作用，抑制RPS25可降低病毒复制能力，提示其作为抗病毒治疗靶点的潜力。
2. **"RPS25 promotes colorectal cancer progression via ribosome biogenesis"**
*作者：Zhang Y, et al. (2021)*
摘要：通过免疫组化(使用RPS25抗体)分析结直肠癌组织，发现RPS25高表达与肿瘤转移和患者不良预后相关，机制涉及核糖体生物合成通路调控。
3. **"Development of a high-affinity monoclonal antibody against human RPS25 for diagnostic applications"**
*作者：Lee J, et al. (2019)*
摘要：报道了一种新型RPS25单克隆抗体的开发与验证，该抗体在Western blot和免疫荧光中表现出高特异性，可用于癌症及病毒感染模型中RPS25蛋白的定量检测。
(注：以上文献为示例性内容，实际引用时请核实具体文献信息。)
The RPS25 antibody is a research tool designed to detect ribosomal protein S25 (RPS25), a component of the 40S subunit of eukaryotic ribosomes. RPS25. encoded by the RPS25 gene, plays a critical role in mRNA decoding during protein synthesis. Beyond its canonical function in translation, emerging studies suggest RPS25 may participate in non-ribosomal biological processes, including viral replication and cellular stress responses. For example, RPS25 was identified as essential for hepatitis C virus (HCV) internal ribosome entry site (IRES)-mediated translation, highlighting its role in viral pathogenesis.
Structurally, RPS25 is a small protein (~13 kDa) containing conserved RNA-binding motifs, facilitating ribosome assembly and mRNA interactions. Antibodies targeting RPS25 are typically produced in immunized hosts (e.g., rabbits or mice) using synthetic peptides or recombinant protein fragments. These antibodies enable applications such as Western blotting, immunofluorescence, and immunohistochemistry to study RPS25 expression, localization, and interactions in cells or tissues.
Recent research has explored RPS25's involvement in diseases, including cancers, where altered ribosome biogenesis (ribosomopathies) is linked to tumorigenesis. Its dysregulation has been observed in certain cancers, potentially serving as a biomarker or therapeutic target. Validation of RPS25 antibodies requires careful optimization due to cross-reactivity risks with other ribosomal proteins. Ongoing studies continue to unravel its extra-ribosomal roles, emphasizing its importance in both basic and translational research contexts.
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