| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PARP; PPOL; ADPRT; ADPRT1; PARP-1; pADPRT-1; PARP1 |
| Entrez GeneID | 142 |
| clone | 7A10 |
| WB Predicted band size | 117kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human PARP, conjugated to KLH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ORC4抗体的3篇代表性文献(注：文献为虚构示例，实际文献需通过学术数据库检索确认):
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1. **标题**: *ORC4 Antibody Characterization in Human Cell Cycle Regulation*
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种特异性识别ORC4蛋白的单克隆抗体，通过免疫印迹和免疫荧光验证其在HeLa细胞中的特异性。结果证实ORC4在G1期富集于复制起点，为研究DNA复制起始机制提供工具。
2. **标题**: *ORC4 Mutations and Meier-Gorlin Syndrome: A Diagnostic Antibody Approach*
**作者**: Brown L, et al.
**摘要**: 利用ORC4抗体对患者成纤维细胞进行蛋白表达分析，发现部分Meier-Gorlin综合征患者存在ORC4表达缺失或异常，提示该抗体在遗传病诊断中的潜在价值。
3. **标题**: *Chromatin Immunoprecipitation Using ORC4 Antibody Reveals Replication Origin Dynamics*
**作者**: Garcia R, et al.
**摘要**: 通过ORC4抗体的染色质免疫沉淀(ChIP)技术，绘制了酿酒酵母基因组中复制起点的动态分布图谱，揭示了ORC4在复制许可中的时空调控机制。
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如需真实文献，建议在PubMed等平台以“ORC4 antibody”或“ORC4 immune localization”为关键词检索。
The ORC4 antibody targets the fourth subunit of the Origin Recognition Complex (ORC), a critical protein complex involved in initiating DNA replication in eukaryotic cells. Discovered in yeast, the ORC (comprising ORC1-6) binds to replication origins during the G1 phase of the cell cycle, serving as a scaffold to recruit CDC6. Cdt1. and the MCM complex, which together assemble the pre-replicative complex. ORC4. an ATPase, plays a key role in stabilizing ORC binding to DNA and coordinating replication licensing.
ORC4 antibodies are essential tools for studying DNA replication mechanisms, cell cycle regulation, and chromatin dynamics. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect ORC4 expression, localization, and interactions. Research has linked ORC4 dysfunction to diseases, including Meier-Gorlin syndrome (a primordial dwarfism disorder) and cancers marked by replication stress.
These antibodies are available as monoclonal or polyclonal forms, often validated for specificity in model organisms like humans, mice, and yeast. Recent studies also explore ORC4's role in epigenetic silencing and heterochromatin formation. As DNA replication fidelity is crucial for genomic stability, ORC4 antibodies remain pivotal in both basic research and clinical investigations of replication-related pathologies.
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