| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PECR |
| Uniprot No | Q9BY49 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-303aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMASWAKGRSYLAPGLLQGQVAIVTGG ATGIGKAIVKELLELGSNVVIASRKLERLKSAADELQANLPPTKQARVIP IQCNIRNEEEVNNLVKSTLDTFGKINFLVNNGGGQFLSPAEHISSKGWHA VLETNLTGTFYMCKAVYSSWMKEHGGSIVNIIVPTKAGFPLAVHSGAARA GVYNLTKSLALEWACSGIRINCVAPGVIYSQTAVENYGSWGQSFFEGSFQ KIPAKRIGVPEEVSSVVCFLLSPAASFITGQSVDVDGGRSLYTHSYEVPD HDNWPKGAGDLSVVKKMKETFKEKAKL |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PECR(Peroxisomal trans-2-enoyl-CoA reductase)重组蛋白的3篇参考文献的简要列举：
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1. **文献名称**: *"Characterization of Recombinant Human Peroxisomal trans-2-Enoyl-CoA Reductase (PECR) and Its Role in Fatty Acid Metabolism"*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人PECR蛋白，分析了其酶活性和在过氧化物酶体脂肪酸延伸途径中的作用，揭示了其在调控细胞脂质代谢中的功能机制。
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2. **文献名称**: *"Structural Insights into PECR: Crystallographic Analysis of a Recombinant Enzyme Involved in Lipid Biosynthesis"*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组PECR蛋白的三维结构，阐明了其底物结合域和催化活性位点的特征，为设计靶向PECR的代谢疾病药物提供了结构基础。
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3. **文献名称**: *"Functional Reconstitution of PECR in Synthetic Lipid Vesicles: Implications for Peroxisomal Redox Balance"*
**作者**: García-Ruiz C, et al.
**摘要**: 利用重组PECR蛋白在人工脂质体中重建其酶活性，证明了PECR在维持过氧化物酶体NAD+/NADH稳态中的关键作用，并探讨了其与氧化应激相关疾病的潜在关联。
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注：以上文献为示例性质，实际引用需根据具体数据库(如PubMed、Web of Science)检索结果调整。若需真实文献，建议以关键词“PECR recombinant protein”或“Peroxisomal trans-2-enoyl-CoA reductase expression”进一步查询。
**Background of PECR Recombinant Protein**
Peroxisomal trans-2-enoyl-CoA reductase (PECR), encoded by the *PECR* gene, is a key enzyme involved in the elongation of fatty acids within peroxisomes. It catalyzes the NADPH-dependent reduction of trans-2-enoyl-CoA intermediates during the final step of the fatty acid elongation cycle, particularly in the synthesis of very long-chain fatty acids (VLCFAs) and polyunsaturated fatty acids (PUFAs). PECR belongs to the short-chain dehydrogenase/reductase (SDR) superfamily and is critical for maintaining lipid homeostasis, membrane integrity, and energy metabolism.
Structurally, PECR contains conserved domains for substrate binding and cofactor interaction, enabling its role in redox reactions. Dysregulation of PECR has been linked to metabolic disorders, including peroxisomal biogenesis defects and conditions associated with impaired fatty acid metabolism, such as neurodegenerative diseases and insulin resistance.
Recombinant PECR protein is produced using expression systems like *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. Its production enables biochemical studies to elucidate enzyme kinetics, substrate specificity, and interactions with regulatory proteins. Recombinant PECR also serves as a tool for screening inhibitors or modulators of fatty acid elongation, offering potential therapeutic avenues for metabolic syndromes.
Research on PECR recombinant protein contributes to understanding peroxisomal lipid metabolism and its broader implications in cellular physiology and disease. Its study bridges gaps in lipid biology, aiding the development of targeted therapies for disorders linked to fatty acid dysregulation.
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