| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | VEAR |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human GGA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GGA2抗体的3篇参考文献的简要列举(内容为模拟示例，非真实文献)：
1. **文献名称**：**"GGA2 regulates BACE1 trafficking and Aβ production through endosomal sorting"**
**作者**：Smith, J. et al.
**摘要**：研究利用GGA2特异性抗体进行免疫印迹和免疫荧光实验，揭示了GGA2在调控β-分泌酶(BACE1)从内体到高尔基体的运输中的作用，并发现其缺失会增加β淀粉样蛋白(Aβ)的生成，提示GGA2在阿尔茨海默病中的潜在病理机制。
2. **文献名称**：**"Monoclonal antibody development for GGA2: A tool to study Golgi-lysosome trafficking"**
**作者**：Chen, L. et al.
**摘要**：报道了一种高特异性的抗GGA2单克隆抗体的开发，通过免疫沉淀和质谱分析验证其可靠性，并证明该抗体可用于追踪GGA2在高尔基体与溶酶体之间的动态定位，为研究囊泡运输提供工具。
3. **文献名称**：**"GGA2-mediated EGFR degradation modulates cancer cell proliferation"**
**作者**：Kim, H. et al.
**摘要**：使用GGA2抗体进行免疫组化和Western blot分析，发现GGA2通过泛素化途径促进表皮生长因子受体(EGFR)的溶酶体降解，抑制GGA2会导致EGFR积累并加速肿瘤细胞增殖。
4. **文献名称**：**"Functional interplay between GGA1 and GGA2 in adaptor protein complexes"**
**作者**：Rodriguez, A. et al.
**摘要**：通过GGA2抗体的共聚焦成像和基因敲除实验，揭示GGA2与GGA1在衔接蛋白复合体中的协同作用，调控细胞内膜运输网络的平衡，并影响神经细胞的功能。
(注：以上文献信息为虚构示例，实际引用需查询真实数据库如PubMed或Google Scholar。)
The GGA2 (Golgi-localized, γ-ear-containing, ARF-binding protein 2) antibody is a tool used to study the GGA2 protein, a member of the GGA family of clathrin adaptors involved in intracellular protein trafficking. GGAs regulate vesicle formation by sorting cargo between the Golgi apparatus, endosomes, and lysosomes. GGA2 contains three functional domains: a VHS domain for cargo recognition (e.g., mannose-6-phosphate receptors), a GAT domain that interacts with ARF GTPases and ubiquitin, and a hinge region connecting to a clathrin-binding γ-adaptin ear (GAE) domain. It plays a key role in maintaining cellular homeostasis by directing lysosomal enzyme transport and modulating receptor recycling/degradation.
GGA2 is implicated in neurodegenerative diseases, such as Alzheimer's, where dysregulation of trafficking pathways (e.g., APP processing) may contribute to pathology. Its expression and activity are also linked to cancer progression and viral infection mechanisms. The GGA2 antibody enables detection and localization of endogenous GGA2 in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies using this antibody have elucidated GGA2's role in COP1 vesicle formation, AP-1 complex interplay, and stress-induced trafficking adaptations. Knockout models reveal compromised lysosomal function, underscoring its physiological significance. Researchers utilize this antibody to explore membrane trafficking dynamics and disease-related dysfunctions.
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