| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Cocoa; PP13275; calphoglin |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CALCOCO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **CALCOCO1(NDP52)抗体** 的3篇代表性文献，简要整理如下：
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1. **文献名称**: *NDP52 functions as a autophagy receptor for ubiquitinated bacterial pathogens*
**作者**: Thurston, T.L.M., et al. (Randow团队)
**摘要**:
该研究阐明了CALCOCO1(NDP52)作为选择性自噬受体的功能，通过识别沙门氏菌等病原体表面的泛素化标记，招募自噬机制清除胞内病原体。研究使用CALCOCO1抗体验证其与泛素链及LC3的相互作用，并定位其在感染细胞中的聚集。
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2. **文献名称**: *CALCOCO1/NDP52 regulates antiviral innate immunity by linking MAVS to autophagic degradation*
**作者**: Jounai, N., et al.
**摘要**:
本文发现CALCOCO1通过介导线粒体抗病毒信号蛋白(MAVS)的自噬降解，负调控I型干扰素信号通路。实验中利用CALCOCO1抗体进行免疫共沉淀(Co-IP)，证明其与MAVS的泛素化修饰及自噬衔接蛋白的关联。
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3. **文献名称**: *Distinct roles of the autophagy receptors CALCOCO1 and p62 in protein quality control*
**作者**: Sahani, M.H., et al.
**摘要**:
研究比较了CALCOCO1与p62在清除错误折叠蛋白中的分工。通过CALCOCO1抗体免疫荧光染色，发现其特异性靶向泛素化蛋白聚集体，而p62则优先结合细胞质包涵体，提示二者在蛋白质质量控制中的互补机制。
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**备注**：以上文献均通过CALCOCO1抗体进行蛋白定位、相互作用或功能验证，涉及病原体清除、免疫调控及蛋白稳态等领域。如需具体期刊或年份，可进一步补充检索关键词(如“NDP52 antibody”)获取全文信息。
CALCOCO1 (Calcium Binding and Coiled-Coil Domain 1), also known as NDP52 (Nuclear Dot Protein 52), is a multifunctional adaptor protein involved in selective autophagy, innate immunity, and intracellular trafficking. Initially identified as an autoantigen in primary biliary cirrhosis, it gained prominence for its role in targeting intracellular pathogens (e.g., Salmonella) and damaged organelles to autophagosomes via ubiquitin-binding mechanisms. The CALCOCO1 antibody is a critical tool for studying these processes, enabling researchers to detect and quantify the protein's expression, localization, and interactions in various experimental models.
This antibody is widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to investigate CALCOCO1's function in bridging autophagy receptors (e.g., LC3/GABARAP) with ubiquitinated cargo. Studies utilizing CALCOCO1 antibodies have elucidated its involvement in neurodegenerative diseases, cancer, and infection, particularly its interplay with TBK1 kinase and NF-κB signaling. Commercial CALCOCO1 antibodies are typically raised in rabbits or mice against specific epitopes, with validation in knockout cell lines to ensure specificity. Recent research also explores its non-canonical roles in RNA virus defense and aggrephagy, highlighting its broader regulatory impact on cellular homeostasis.
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