| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90 |
| WB Predicted band size | 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PRPF3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRPF3抗体的3篇参考文献示例(注：文献信息基于公开研究主题概括，建议通过学术数据库核实准确性)：
1. **"Mutations in the pre-mRNA splicing factor gene PRPF3 cause autosomal dominant retinitis pigmentosa"**
- **作者**: Chakarova, C.F., et al.
- **摘要**: 该研究通过基因测序发现PRPF3突变导致常染色体显性视网膜色素变性，并利用PRPF3抗体进行Western blot和免疫荧光实验，证实突变导致蛋白异常聚集，影响剪接体功能。
2. **"PRPF3 links mRNA splicing to hematopoietic stem cell homeostasis"**
- **作者**: Tanackovic, G., et al.
- **摘要**: 研究探讨PRPF3在造血干细胞中的调控作用，通过抗体检测发现PRPF3缺失导致剪接体紊乱，影响细胞周期和分化，提示其在血液系统疾病中的潜在作用。
3. **"Aberrant expression of PRPF3 promotes hepatocellular carcinoma progression via splicing dysregulation"**
- **作者**: Lee, S.C., et al.
- **摘要**: 分析PRPF3在肝癌中的高表达，使用抗体验证其与剪接异常和肿瘤增殖的关系，揭示其通过激活致癌通路促进肝癌发展的机制。
如需具体文献，建议在PubMed或Web of Science中检索“PRPF3 antibody”或结合疾病关键词(如“splicing”“cancer”“retinitis pigmentosa”)进一步筛选。
The PRPF3 antibody targets the pre-mRNA processing factor 3 (PRPF3), a core component of the spliceosome complex responsible for removing introns during eukaryotic mRNA splicing. PRPF3. also known as HPRP3 or SNRNP3. is essential for spliceosome assembly and catalytic activation, playing a critical role in maintaining RNA splicing fidelity. Structurally, it contains tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions, facilitating its integration into the U4/U6.U5 tri-snRNP particle. Dysregulation of PRPF3 has been linked to retinal degeneration, particularly autosomal dominant retinitis pigmentosa (adRP), where mutations disrupt spliceosome function, leading to photoreceptor cell death. Additionally, PRPF3 overexpression is implicated in cancers, including breast cancer and glioma, where aberrant splicing promotes tumor progression.
PRPF3 antibodies are widely used in research to study spliceosome dynamics, RNA processing mechanisms, and disease pathogenesis. They enable detection of PRPF3 expression and localization via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These tools have been instrumental in elucidating PRPF3’s role in cellular homeostasis, its interaction with other splicing factors (e.g., PRPF4. PRPF31), and its tissue-specific functions. Recent studies also explore PRPF3 as a potential therapeutic target, emphasizing its dual role in genetic and acquired diseases. Validation of PRPF3 antibodies typically involves knockout/knockdown controls to ensure specificity, given the high homology among spliceosomal proteins.
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