首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子补体片断5a(C5a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5a含量。C5a是补体系统激活过程中产生的重要炎症介质。其背景在于补体系统是机体免疫防御的关键部分。研究机制方面，C5a可与受体结合，引发炎症细胞趋化、活化，放大炎症反应，在感染、自身免疫病等多种疾病病理进程中发挥重要作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，科研人员可将其应用于炎症信号通路研究、免疫调控机制探索、疾病动物模型评估（如感染性疾病或自身免疫模型），以及抗炎药物研发过程中生物标志物的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C5a
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of rabbit C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-93 | ||||
| EDTA plasma (n=4) | 101 | 97-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.8212.9882.9052.813202.5642.4142.4892.397101.7671.7461.7571.66550.9910.9740.9830.8912.50.4840.4960.4900.3981.250.2860.2970.2920.2000.6250.1570.1620.1600.06800.0910.0930.092 | ||||||
货期：3-5 working days
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