| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TPARM |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TTC12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及TTC12抗体的文献示例(注：文献为虚拟示例，实际需根据具体研究验证)：
1. **文献名称**: "TTC12 mutations cause primary ciliary dyskinesia by disrupting ciliary localization"
**作者**: Smith A, et al.
**摘要**: 研究通过免疫荧光和Western blot技术，利用TTC12抗体揭示了TTC12蛋白在纤毛基部的定位功能，发现其基因突变导致纤毛运动障碍的机制。
2. **文献名称**: "TTC12 as a schizophrenia risk gene: Expression analysis in postmortem brain tissue"
**作者**: Chen L, et al.
**摘要**: 通过TTC12抗体检测人脑组织样本，发现TTC12在精神分裂症患者前额叶皮层中的表达显著降低，提示其与多巴胺信号通路的潜在关联。
3. **文献名称**: "TTC12 interacts with DNAH1 and regulates cancer cell migration"
**作者**: Wang Y, et al.
**摘要**: 使用TTC12抗体进行免疫共沉淀实验，证实TTC12与动力蛋白DNAH1的相互作用，并发现其过表达促进肺癌细胞迁移。
4. **文献名称**: "Antibody validation for TTC12 in zebrafish embryonic development"
**作者**: Gomez R, et al.
**摘要**: 研究验证了TTC12抗体在斑马鱼模型中的特异性，发现TTC12缺失导致胚胎纤毛发育异常，支持其在早期发育中的关键作用。
(注：以上文献为示例性内容，实际研究需查阅PubMed等数据库获取准确信息。)
The TTC12 antibody is a research tool designed to detect the TTC12 (tetratricopeptide repeat domain 12) protein, a component critical for cytoplasmic pre-assembly of dynein arms in motile cilia. TTC12 contains tetratricopeptide repeat (TPR) motifs, which mediate protein-protein interactions, and is part of a complex with DNAAF1 and DNAAF3. facilitating the cytoplasmic assembly of dynein motor proteins before their transport to cilia. Mutations in TTC12 are linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and infertility due to dysfunctional cilia and flagella.
TTC12 antibodies are typically produced in rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry to study TTC12 expression, localization, and interactions in cell lines or tissues. Specificity is often confirmed via knockout controls or siRNA knockdown. These antibodies have been instrumental in elucidating TTC12's role in dynein arm biogenesis and its association with ciliopathies. Recent studies also explore TTC12's potential involvement in sperm development and respiratory epithelial function, highlighting its broader relevance in cellular dynamics and disease mechanisms.
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