首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体蛋白5(C5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5含量。C5是补体系统中的关键成分。其背景在于补体系统是固有免疫重要部分，C5在其中起着枢纽作用。研究机制上，C5可被激活裂解成C5a和C5b，C5a参与炎症反应，C5b可启动膜攻击复合物形成，参与免疫防御及相关疾病发生发展。试剂盒检测范围为0.25 ng/mL-16 ng/mL，适用于研究感染、自身免疫性疾病或炎症模型中C5的动态变化，为探索补体系统相关机制及药物干预效果提供可靠工具，适用于实验室批量检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C5 ELISA Kit; HcComplement C5 ELISA Kit; Hemolytic complement) [Cleaved into: Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain] ELISA Kit
缩写：C5
Uniprot No.：P06684
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 ng/mL-16 ng/mL
灵敏度：0.06 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 93 | ||||
| Range % | 86-100 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 87-106 | |||||
| 1:400 | Average % | 90 | ||||
| Range % | 86-99 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 90-105 | |||||
回收率：
| The recovery of mouse C5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 87-101 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected162.0111.9641.9881.82781.5891.5641.5771.41641.0951.0381.0670.90620.6430.6200.6320.47110.4420.4260.4340.2730.50.2970.2850.2910.1300.250.2210.2160.2190.05800.1560.1650.161 | ||||||
货期：3-5 working days
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