| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MRT15; ERMAN1; ERManI; MANA-ER |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAN1B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MAN1B1抗体的代表性文献摘要(虚构示例，仅供参考)：
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1. **文献名称**: *MAN1B1 as a Biomarker for Hepatocellular Carcinoma: Expression Analysis and Clinical Implications*
**作者**: Zhang L, et al.
**摘要**: 该研究通过免疫组化(使用特异性MAN1B1抗体)发现，MAN1B1在肝癌组织中显著高表达，并与患者不良预后相关，提示其可能作为肝癌诊断和治疗靶点。
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2. **文献名称**: *Structural Insights into MAN1B1 Enzyme Function through Cryo-EM*
**作者**: Hansen T, et al.
**摘要**: 本研究利用抗MAN1B1抗体进行蛋白纯化及定位，结合冷冻电镜解析了MAN1B1的三维结构，揭示了其参与内质网糖蛋白质量控制的分子机制。
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3. **文献名称**: *MAN1B1 Deficiency Disrupts ERAD Pathway in Cellular Models*
**作者**: Avezov E, et al.
**摘要**: 通过Western blot和免疫荧光(使用MAN1B1抗体)发现，MAN1B1缺失导致内质网相关降解(ERAD)功能异常，引发错误折叠蛋白积累，证实其在细胞蛋白稳态中的关键作用。
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注：以上文献信息为示例性质，实际引用时需查询真实数据库(如PubMed)获取准确信息。
MAN1B1 (Mannosidase Alpha Class 1B Member 1), also known as ERManI, is a Golgi-resident enzyme belonging to glycosyl hydrolase family 47. It catalyzes the trimming of mannose residues from N-linked glycans during protein folding, playing a critical role in endoplasmic reticulum-associated degradation (ERAD) by monitoring glycoprotein quality and directing misfolded proteins to proteasomal degradation. Dysregulation of MAN1B1 is linked to congenital disorders of glycosylation (CDGs), cancer progression, and metabolic diseases, highlighting its importance in cellular homeostasis.
MAN1B1 antibodies are essential tools for studying its expression, localization, and function. These antibodies, typically raised in rabbits or mice, target specific epitopes of the MAN1B1 protein and are validated for applications like Western blotting, immunofluorescence, and immunohistochemistry. High-quality antibodies exhibit specificity for MAN1B1 over related mannosidases (e.g., MAN1A1/A2), enabling precise detection in cell lysates or tissue samples. Recent studies using MAN1B1 antibodies have uncovered its roles in viral entry, immune response modulation, and cancer metastasis, underscoring its therapeutic potential. However, variability in antibody performance across experimental conditions necessitates careful validation. Researchers rely on these reagents to explore MAN1B1’s involvement in glycoprotein quality control pathways and disease mechanisms.
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