Rabbit Polyclonal RBM15 Antibody

The RNA-binding motif protein 15 (RBM15), also known as OTT1. is a nuclear protein involved in mRNA processing, including splicing, export, and epigenetic regulation. It contains an N-terminal RNA recognition motif (RRM) and C-terminal SPOC (SPEN paralog and ortholog C-terminal) domain, enabling interactions with RNA, transcription factors, and chromatin modifiers. RBM15 forms heterodimers with RBM15B (OTT3) to regulate hematopoietic and neural development. Dysregulation of RBM15. particularly through chromosomal translocations (e.g., RBM15-MKL1 in acute megakaryoblastic leukemia), is linked to oncogenesis. Antibodies targeting RBM15 are critical tools for studying its role in normal cellular functions (e.g., X chromosome inactivation, Notch signaling) and disease mechanisms. These antibodies are commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect RBM15 expression, localization, and interactions. Validated antibodies often undergo specificity testing via knockout controls. Commercial RBM15 antibodies are typically raised against epitopes within its N-terminal RRM or C-terminal SPOC domains. Researchers utilize these reagents to explore RBM15's involvement in viral RNA regulation, hematopoiesis, and cancer biology, particularly in leukemia models.