关键信息
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基因名
PRAK/MAPKAPK5
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简介
PRAK/MAPKAPK5 protein, a serine/threonine kinase, responds to cellular stress and pro-inflammatory cytokines and acts as a tumor suppressor through MAP kinases (including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta) activated by phosphorylation. Initially located in the nucleus, upon activation, it moves to the cytoplasm and phosphorylates the heat shock protein HSP27. PRAK/MAPKAPK5 Protein, Human (sf9, His-GST) is the recombinant human-derived PRAK/MAPKAPK5 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- 应用
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别名
MAP kinase-activated protein kinase 5; MAPKAP-K5; MK5; PRAK
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种属
Human
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表达系统
Baculovirus
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标签
N-GST;N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IW41-2
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表达区间
M1-Q471
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蛋白长度
Partial
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分子量
35-45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PRAK (p38 MAPK-activated protein kinase) is a serine/threonine kinase that is primarily activated by the p38 mitogen-activated protein kinase (MAPK) pathway, which plays a crucial role in cellular responses to stress, inflammation, and differentiation. MAPKAPK5, also known as PRAK, is involved in various cellular processes, including cell proliferation, survival, and apoptosis. Research has shown that PRAK/MAPKAPK5 can influence the activity of several transcription factors and signaling pathways, thereby regulating gene expression in response to environmental stimuli. Given its integral role in the MAPK signaling cascade, PRAK has become a focal point in cancer research, as it can modulate tumor growth and metastasis. Understanding the structure and function of PRAK/MAPKAPK5 through the study of recombinant proteins offers valuable insights into its mechanistic role in disease states. This research can also pave the way for the development of therapeutic strategies targeting the MAPK pathway, which is often dysregulated in various cancers. As such, the recombinant expression and characterization of PRAK/MAPKAPK5 is vital for elucidating its biochemical properties, interactions with other cellular proteins, and potential as a therapeutic target in oncology.
