| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human WDR47 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是为您整理的3-4篇关于WDR47抗体的模拟参考文献(注：以下内容为示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *WDR47 regulates neuronal migration and cortical development through APC-mediated microtubule dynamics*
**作者**: Tanaka, K. et al. (2015)
**摘要**: 本研究利用WDR47特异性抗体，揭示了WDR47通过与腺瘤性息肉病蛋白(APC)相互作用调控微管稳定性，影响神经元迁移和大脑皮层发育的机制。抗体验证包括免疫印迹和免疫组化实验。
2. **文献名称**: *Characterization of WDR47 as a novel interactor of the Reelin signaling pathway*
**作者**: Smith, L.M. et al. (2018)
**摘要**: 文章报道了WDR47在Reelin信号通路中的作用，通过免疫共沉淀(Co-IP)和WDR47抗体验证其与Dab1蛋白的关联，提示其在神经发育障碍中的潜在病理意义。
3. **文献名称**: *Antibody-based profiling of WDR47 in postmortem brain tissues of schizophrenia patients*
**作者**: Chen, X. et al. (2020)
**摘要**: 使用WDR47抗体对比精神分裂症患者与健康人脑组织样本，发现WDR47蛋白表达水平异常，提示其可能通过突触可塑性参与精神疾病发生。
4. **文献名称**: *WDR47 modulates tau phosphorylation and aggregation in Alzheimer’s disease models*
**作者**: Gupta, R. & Lee, J. (2022)
**摘要**: 研究通过siRNA敲低和WDR47抗体检测，证明WDR47通过GSK3β调控tau蛋白磷酸化，可能成为阿尔茨海默病治疗的新靶点。
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**注意**：以上文献为示例性内容，实际研究请通过 **PubMed** 或 **Google Scholar** 检索关键词“WDR47 antibody”或“WDR47 function”获取真实文献。建议优先选择近5年内的研究，并关注《Journal of Neuroscience》《Cell Reports》等期刊。
**Background of WDR47 Antibody**
The WDR47 (WD Repeat Domain 47) protein, also known as CAKA or KIAA0892. is a member of the WD40-repeat protein family, which plays critical roles in diverse cellular processes, including signal transduction, autophagy, and cytoskeletal organization. Structurally, WDR47 contains seven WD40 repeats that facilitate protein-protein interactions, enabling its involvement in multiprotein complexes. It is particularly noted for its interaction with microtubules and participation in neuronal development, where it regulates axonal outgrowth and guidance by modulating microtubule stability.
WDR47 antibodies are essential tools for studying the protein’s expression, localization, and functional mechanisms. These antibodies are commonly generated in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of human or mouse WDR47. Validated applications include Western blotting, immunofluorescence, and immunoprecipitation, allowing researchers to investigate WDR47’s role in neurodevelopmental disorders, cancer, and neurodegenerative diseases. For instance, studies link WDR47 dysregulation to glioblastoma progression and intellectual disability.
High-quality WDR47 antibodies undergo rigorous validation for specificity (e.g., knockout cell controls) and reproducibility. Commercial antibodies often provide datasheets detailing their reactivity across species (human, mouse, rat) and recommended experimental conditions. Researchers prioritize these reagents to explore WDR47’s interplay with pathways like the PI3K/AKT/mTOR axis or its association with autophagy-related proteins, advancing insights into cellular homeostasis and disease mechanisms.
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