| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TF2A2; TFIIA; T18745; TFIIAS; HsT18745; TFIIA-12; TFIIA-gamma |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GTF2A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GTF2A2抗体的3篇参考文献示例(注：部分内容为模拟虚构，仅供参考)：
1. **"Development and validation of a specific polyclonal antibody for human GTF2A2"**
*作者：Li, X. et al.*
摘要：本研究开发了一种针对人源GTF2A2蛋白的多克隆抗体，通过Western blot和免疫荧光验证其特异性，证实该抗体可用于检测细胞核内GTF2A2的表达水平，并应用于转录机制研究。
2. **"GTF2A2 modulates RNA polymerase III transcription and is implicated in tumor progression"**
*作者：Chen, J. & Wang, Y.*
摘要：文章利用抗GTF2A2抗体进行染色质免疫沉淀(ChIP)，发现GTF2A2参与RNA聚合酶III的转录调控，并在癌症组织中表达异常，提示其可能作为肿瘤生物标志物。
3. **"Characterization of GTF2A2 interactions in the TFIIA complex using epitope-specific antibodies"**
*作者：Smith, R. et al.*
摘要：通过表位特异性抗体分析GTF2A2在TFIIA复合体中的相互作用，揭示其与GTF2A1的协同作用机制，为转录起始复合体的结构研究提供工具。
(注：若需真实文献，建议在PubMed或Web of Science中搜索“GTF2A2 antibody”结合具体研究领域筛选。)
The GTF2A2 antibody targets the General Transcription Factor IIA subunit 2 (GTF2A2), a key component of the RNA polymerase II transcription machinery. GTF2A2. along with GTF2A1. forms the heterodimeric Transcription Factor IIA (TFIIA), which stabilizes the binding of TATA-binding protein (TBP) to DNA and enhances the assembly of the pre-initiation complex (PIC) during transcription initiation. This process is critical for regulating the expression of protein-coding genes. GTF2A2 is ubiquitously expressed and plays a role in cellular processes such as differentiation, development, and stress responses.
Antibodies against GTF2A2 are widely used in molecular biology research to study transcriptional regulation, protein-protein interactions, and TFIIA’s structural or functional roles in disease contexts. They are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and complex formation. Commercially available GTF2A2 antibodies are typically validated for specificity using knockout/knockdown controls or peptide-blocking assays.
Dysregulation of TFIIA has been implicated in cancers and neurological disorders, making GTF2A2 a potential biomarker or therapeutic target. Research utilizing these antibodies has provided insights into transcriptional anomalies in diseases and basic gene regulation mechanisms. However, variability in antibody performance across experimental conditions necessitates careful optimization and validation in specific applications.
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