| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MTHFD2L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MTHFD2L抗体的3篇参考文献及其摘要内容：
1. **"Mitochondrial methylenetetrahydrofolate dehydrogenase 2-like enzyme (MTHFD2L) supports cancer cell proliferation by regulating one-carbon metabolism"**
- **作者**: Koufaris et al.
- **摘要**: 该研究揭示了MTHFD2L在线粒体一碳代谢中的作用，利用特异性抗体验证其在多种癌细胞中的高表达，并证明其通过调控叶酸代谢促进肿瘤生长。
2. **"Development and characterization of a polyclonal antibody targeting human MTHFD2L for functional studies in embryonic development"**
- **作者**: Smith et al.
- **摘要**: 本文报道了一种针对人MTHFD2L蛋白的多克隆抗体的开发，通过免疫印迹和免疫荧光技术验证其特异性，并用于研究该酶在小鼠胚胎发育中的表达模式。
3. **"MTHFD2L as a potential therapeutic target in colorectal cancer: Antibody-based inhibition suppresses tumor progression"**
- **作者**: Chen et al.
- **摘要**: 研究通过MTHFD2L抗体抑制实验，证明靶向该酶可减少结直肠癌细胞增殖和转移，抗体被用于检测患者组织中MTHFD2L的异常高表达。
注：以上文献为示例，实际引用时需以真实发表的论文为准。若研究较少，建议扩展检索范围至MTHFD2L的功能研究或相关抗体技术文献。
The MTHFD2L (methylenetetrahydrofolate dehydrogenase 2-like) antibody is a tool used to detect and study the MTHFD2L protein, a mitochondrial enzyme involved in one-carbon metabolism. This metabolic pathway is critical for nucleotide synthesis, methylation reactions, and cellular redox balance. MTHFD2L shares homology with MTHFD2. another mitochondrial folate enzyme, but exhibits distinct functional roles. While MTHFD2 is linked to NADPH production and proliferating cells, MTHFD2L is proposed to function in NAD-dependent reactions, though its precise biological mechanisms remain under investigation.
Research highlights MTHFD2L's potential role in cancer, as its expression is upregulated in certain tumors, including gliomas and lung cancers, correlating with poor prognosis. This makes it a candidate biomarker or therapeutic target. The antibody enables detection of MTHFD2L in techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF), aiding in studies of its tissue distribution, subcellular localization, and expression patterns under varying physiological or pathological conditions.
Commercial MTHFD2L antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated silencing. Some cross-reactivity concerns exist due to structural similarities with MTHFD2. emphasizing the need for rigorous validation in experimental models. Its study contributes to understanding mitochondrial one-carbon metabolism dysregulation in diseases, particularly cancer, and supports drug discovery efforts targeting folate-dependent enzymes.
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