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Human Calreticulin,CRT ELISA Kit

  • 中文名： 人钙网蛋白(CRT)ELISA Kit
货号: IPD-E09787h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人钙网蛋白(CRT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液,组织匀浆样本中的CALR含量。CALR是一个重要靶点，它是内质网伴侣蛋白。研究发现其突变与骨髓增殖性肿瘤密切相关，突变体CALR能激活下游信号通路，影响细胞的增殖、分化等生物学行为。目前围绕CALR开展的研究有助于为相关疾病的治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于科研领域中对CRT蛋白表达水平的精准定量分析，例如在癌症生物学研究中探索肿瘤细胞应激反应机制，或在免疫学实验中评估炎症条件下CRT的分泌动态。实验操作兼容多种生物样本，尤其适合同时开展跨样本类型的蛋白表达对比研究，为揭示CRT在疾病模型中的分子调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Autoantigen RO ELISA Kit; CALR ELISA Kit; CALR protein ELISA Kit; CALR_HUMAN ELISA Kit; Calregulin ELISA Kit; Calreticulin ELISA Kit; cC1qR ELISA Kit; CRP55 ELISA Kit; CRT ELISA Kit; CRTC ELISA Kit; Endoplasmic reticulum resident protein 60 ELISA Kit; Epididymis secretory sperm binding protein Li 99n ELISA Kit; ERp60 ELISA Kit; FLJ26680 ELISA Kit; grp60 ELISA Kit; HACBP ELISA Kit; HEL S 99n ELISA Kit; RO ELISA Kit; Sicca syndrome antigen A (autoantigen Ro; calreticulin) ELISA Kit; Sicca syndrome antigen A ELISA Kit; SSA ELISA Kit

  • 缩写：CALR

  • Uniprot No.：P27797

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, urine,tissue homogenates

  • 检测范围：0.156 ng/mL-10 ng/mL

  • 灵敏度：0.039 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Tags & Cell Markers

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %93
    Range %89-98
    1:4Average %90
    Range %85-95
    1:8Average %104
    Range %100-110

  • 回收率：

    The recovery of human CRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)8480-89
    EDTA plasma (n=4)8984-96














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected102.6082.5722.5902.37052.1722.0722.1221.9022.51.5111.5631.5371.3171.251.1061.0931.1000.8800.6250.6330.5970.6150.3950.3120.4290.4640.4470.2270.1560.3220.3100.3160.09600.2210.2190.220　





  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CRT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled CRT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

    • An incubator can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：3-5 working days

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