| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | WDR32 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DCAF10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DCAF10抗体的3篇参考文献(内容基于模拟生成，非真实文献)：
1. **文献名称**：*DCAF10 as a substrate receptor for CUL4-DDB1 ubiquitin ligase: Antibody development and functional characterization*
**作者**：Smith J, et al.
**摘要**：该研究开发了针对DCAF10蛋白的高特异性多克隆抗体，验证了其在Western blot和免疫沉淀实验中的应用，并揭示DCAF10通过CUL4-DDB1复合物调控底物泛素化降解的分子机制。
2. **文献名称**：*DCAF10 antibody-based profiling reveals tissue-specific expression patterns in cancer*
**作者**：Li X, et al.
**摘要**：利用商业化DCAF10抗体进行免疫组化分析，发现DCAF10在肺癌和结直肠癌组织中表达显著上调，且与患者预后不良相关，提示其可能作为癌症生物标志物。
3. **文献名称**：*Structural insights into DCAF10 interaction with viral proteins using cryo-EM*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜解析DCAF10与病毒蛋白的互作结构，研究中采用特异性抗体纯化内源性DCAF10复合物，证实其在宿主抗病毒反应中的调控作用。
4. **文献名称**：*DCAF10 knockdown via antibody-mediated targeting inhibits proliferation in hepatocellular carcinoma*
**作者**：Chen R, et al.
**摘要**：开发了靶向DCAF10的功能阻断性单克隆抗体，体外实验表明其可抑制肝癌细胞增殖，为靶向泛素化通路的治疗策略提供依据。
(注：以上文献为示例，实际研究中请通过PubMed或专业数据库检索真实发表文献。)
The DCAF10 (DDB1- and CUL4-associated factor 10) antibody is a research tool used to study the DCAF10 protein, a member of the WD40 repeat-containing DCAF family. DCAF10 serves as a substrate receptor within the CUL4-DDB1 E3 ubiquitin ligase complex (CRL4), which plays a critical role in ubiquitination-mediated protein degradation. This protein is involved in recruiting specific substrates for polyubiquitination, marking them for proteasomal destruction. DCAF10's interactions are linked to cellular processes such as DNA repair, cell cycle regulation, and epigenetic modulation, though its exact biological functions remain under investigation.
Antibodies targeting DCAF10 enable researchers to detect, quantify, or localize the protein in various experimental models (e.g., Western blot, immunofluorescence, IP). They are essential for exploring DCAF10's role in diseases, particularly cancer, where dysregulation of ubiquitin-proteasome pathways is common. Recent studies suggest DCAF10 may influence oncogenic signaling or drug resistance, making it a potential therapeutic target. However, detailed mechanistic insights and clinical relevance require further validation. Commercial DCAF10 antibodies are typically validated for specificity and affinity, though performance may vary across applications. Ongoing research aims to clarify its interactome and regulatory networks in cellular homeostasis and pathology.
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