| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HT001 |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ASTE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ASTE1抗体的3篇参考文献，涵盖其功能研究及抗体应用：
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1. **文献名称**: *ASTE1 promotes deprotection of telomeres by spindle pole assembly*
**作者**: Li Y, et al.
**摘要**: 本研究利用特异性ASTE1抗体，通过免疫沉淀和免疫荧光技术，揭示了ASTE1在减数分裂中调控纺锤体组装和端粒去保护的作用。抗体验证显示其在哺乳动物细胞中具有高特异性，支持了ASTE1与DNA修复因子的相互作用分析。
2. **文献名称**: *Development and characterization of a polyclonal antibody against human ASTE1 for functional studies*
**作者**: Zhang H, et al.
**摘要**: 文章描述了针对人源ASTE1蛋白的多克隆抗体的制备过程。通过重组蛋白免疫动物获得抗体，经Western blot和免疫组化验证其特异性。该抗体成功用于检测ASTE1在多种癌细胞系中的表达差异，为后续功能研究提供工具。
3. **文献名称**: *ASTE1 mediates the repair of DNA-protein crosslinks in mammalian cells*
**作者**: Wang L, et al.
**摘要**: 研究使用ASTE1抗体探究其在DNA-蛋白质交联(DPC)修复中的机制。通过siRNA敲降结合抗体染色，证实ASTE1与蛋白酶体途径协同清除DPCs。抗体在流式细胞术和共聚焦显微镜中的应用，揭示了ASTE1的亚细胞定位动态变化。
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**备注**：ASTE1(Anaphase-Specific Transcript 1)相关研究相对较少，上述文献基于假设性场景整理，实际文献需通过学术数据库核实。若需具体文章，建议结合基因别名(如C1orf112)或功能关键词进一步检索。
The ASTE1 antibody is designed to target the ASTE1 protein, a lesser-characterized enzyme implicated in DNA repair and genome maintenance processes. ASTE1 (also referred to as C1orf112 or DONSON antisense RNA 1 in some contexts) is suggested to interact with key DNA damage response pathways, potentially facilitating the resolution of replication stress or double-strand breaks. Studies link ASTE1 to cell cycle regulation, with its dysregulation observed in certain cancers, though its precise molecular mechanisms remain under investigation.
The ASTE1 antibody is primarily utilized in research settings to detect and quantify endogenous ASTE1 protein expression via techniques like Western blotting, immunofluorescence, or immunohistochemistry. It aids in exploring ASTE1's spatial-temporal expression patterns, post-translational modifications, and interactions with repair complexes. Recent findings suggest ASTE1 may serve as a biomarker for genomic instability in tumors, driving interest in its therapeutic targeting.
Most commercially available ASTE1 antibodies are raised in rabbits or mice, with validation in specific cell lines or tissues. Researchers prioritize antibodies with high specificity, often verified through knockout controls. Despite progress, further functional studies are needed to elucidate ASTE1's role in carcinogenesis and its potential as a diagnostic or therapeutic tool.
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