| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MES; MKS; BBS13; POC12; JBTS28 |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MKS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MKS1抗体的示例参考文献(内容为模拟，仅供参考)：
1. **标题**：*MKS1 mutations cause ciliary transport defects in Meckel syndrome*
**作者**：Smith JD et al.
**摘要**：研究通过免疫荧光和Western blot分析，使用MKS1抗体证实了MKS1蛋白在纤毛基体的定位异常，突变导致纤毛相关蛋白运输障碍，揭示了其在Meckel综合征中的致病机制。
2. **标题**：*Interaction of MKS1 with B9D1/B9D2 in ciliogenesis*
**作者**：Andersen JS et al.
**摘要**：通过免疫共沉淀(Co-IP)和抗体标记实验，发现MKS1与B9D1/B9D2形成复合物，调控纤毛形成，抗体验证了其在细胞中的共定位及功能关联。
3. **标题**：*MKS1 antibody reveals tissue-specific expression in murine models*
**作者**：Garcia-Gonzalo FR et al.
**摘要**：利用MKS1抗体进行组织染色，发现小鼠肾脏、神经管等组织中MKS1高表达，敲除模型显示其与多囊肾及神经管缺陷相关。
4. **标题**：*MKS1 and TCTN2 co-localize in ciliary transition zone*
**作者**：Chih B et al.
**摘要**：通过抗体共标记和超分辨成像，证实MKS1与TCTN2在纤毛过渡区协同作用，维持纤毛膜结构完整性。
**注意**：以上为示例，实际文献需通过PubMed或Google Scholar检索关键词“MKS1 antibody”或“MKS1 function”获取。
The MKS1 antibody is a crucial tool in studying ciliopathies, particularly Meckel syndrome (MKS), a lethal autosomal recessive disorder. MKS1. a gene located on chromosome 17q22. encodes a component of the transition zone in primary cilia, essential for ciliary function and signaling. Mutations in MKS1 disrupt ciliogenesis, leading to multi-organ defects (e.g., renal cysts, polydactyly, CNS malformations).
The MKS1 antibody targets the MKS1 protein (∼70 kDa), enabling detection in tissues/cells via Western blot, immunofluorescence, or immunohistochemistry. It aids in elucidating MKS1's role in ciliary assembly, Hedgehog signaling, and interactions with other ciliary proteins (e.g., B9 proteins, TMEM67). Researchers use it to model MKS pathogenesis, validate gene-editing outcomes, and explore therapeutic targets. Commercially available from suppliers like Sigma-Aldrich and Abcam, it’s typically validated in knockout controls. Its applications extend to related ciliopathies (e.g., Bardet-Biedl syndrome, Joubert syndrome), emphasizing its importance in developmental biology and genetic disorder research. Proper validation remains critical due to potential cross-reactivity with homologous proteins.
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