| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GIG13; FASPS3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PER3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及PER3抗体的研究文献概览：
1. **文献名称**: "PER3 expression in human breast cancer and its association with clinicopathological parameters"
**作者**: Zhao et al. (2015)
**摘要**: 通过免疫组化(使用PER3特异性抗体)分析乳腺癌组织中PER3蛋白的表达水平，发现低表达与肿瘤恶性程度高及患者预后不良相关，提示PER3可能作为抑癌基因发挥作用。
2. **文献名称**: "The role of PER3 polymorphism in human circadian rhythms and sleep regulation"
**作者**: Archer et al. (2012)
**摘要**: 研究PER3基因多态性对睡眠相位障碍的影响，利用Western blot(PER3抗体)检测不同基因型个体的成纤维细胞中蛋白表达差异，发现特定变异与PER3蛋白稳定性降低相关。
3. **文献名称**: "PER3 protein expression in colorectal cancer and its correlation with survival"
**作者**: Knutsson et al. (2007)
**摘要**: 采用免疫荧光和ELISA(基于PER3抗体)检测结直肠癌组织样本，发现PER3表达缺失与肿瘤转移风险升高及患者生存期缩短显著相关。
4. **文献名称**: "Tissue-specific expression of PER3 in human peripheral tissues"
**作者**: von Schantz et al. (2004)
**摘要**: 通过免疫印迹和免疫组化(使用兔源PER3多克隆抗体)，首次系统描述PER3蛋白在人体多种外周组织中的昼夜表达模式，揭示其与中央生物钟的调控关联。
注：以上内容为示例性概括，具体文献需通过学术数据库(如PubMed)核实。
The PER3 antibody is a crucial tool in circadian rhythm research, targeting the Period Circadian Regulator 3 (PER3) protein, a core component of the molecular clock machinery. PER3. encoded by the *PER3* gene, forms part of the PER family (PER1-3) that interacts with CRY proteins to inhibit CLOCK-BMAL1 transcriptional activity, thereby regulating circadian oscillations. It plays a key role in maintaining circadian periodicity, sleep-wake cycles, and metabolic homeostasis.
PER3 antibodies are widely used to detect PER3 expression in tissues (e.g., brain, liver) via techniques like Western blot, immunohistochemistry, and immunofluorescence. These antibodies help investigate PER3’s temporal expression patterns, post-translational modifications (e.g., phosphorylation), and interactions with other clock proteins. Research highlights its importance in disorders like delayed sleep phase syndrome, depression, and cancer, where circadian disruption is implicated.
Species-specific PER3 antibodies (human, mouse, rat) are available, with validation emphasizing specificity to avoid cross-reactivity with PER1/PER2. Some antibodies distinguish between PER3 isoforms arising from alternative splicing. Variants like the human PER3^4/4 polymorphism, linked to altered sleep behaviors, are also studied using isoform-targeted antibodies. Recent work explores PER3’s extra-circadian roles, including DNA damage response and tumor suppression, expanding the antibody’s utility in interdisciplinary research.
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