| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | S14; EMTB |
| WB Predicted band size | 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS14 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于RPS14抗体的3-4篇文献示例及其摘要概括：
1. **文献名称**：*RPS14 Deficiency as a Therapeutic Target in Myelodysplastic Syndromes*
**作者**：Ebert BL, et al.
**摘要**：研究揭示了RPS14基因在5q-综合征(一种骨髓增生异常综合征)中的关键作用，利用RPS14抗体进行蛋白质敲低实验，发现其缺失导致红细胞生成障碍，为靶向治疗提供了依据。
2. **文献名称**：*Ribosomal Protein S14 Modulates p53 Activity in Colorectal Cancer*
**作者**：Zhou X, et al.
**摘要**：通过RPS14抗体检测肿瘤组织中RPS14的表达水平，发现RPS14下调通过影响核糖体生物合成间接激活p53通路，抑制结直肠癌细胞增殖。
3. **文献名称**：*Functional Analysis of RPS14 in Zebrafish Hematopoiesis*
**作者**：Danilova N, et al.
**摘要**：利用RPS14抗体定位斑马鱼模型中RPS14的表达，证明其缺失导致类似人类贫血的表型，强调了其在脊椎动物造血系统中的保守功能。
4. **文献名称**：*RPS14 Antibody-Based Detection in Gene Therapy Monitoring*
**作者**：Li J, et al.
**摘要**：开发了一种高特异性RPS14抗体，用于监测基因治疗中RPS14的恢复表达，验证其在逆转5q-综合征患者细胞表型中的疗效。
(注：以上文献为示例性内容，实际引用需根据具体研究检索真实数据库。)
The ribosomal protein S14 (RPS14) is a critical component of the 40S ribosomal subunit, playing a vital role in rRNA processing and ribosome assembly. Antibodies targeting RPS14 are widely used in research to investigate ribosome biogenesis, protein synthesis regulation, and its association with human diseases. RPS14 gained prominence due to its link to 5q- syndrome, a subtype of myelodysplastic syndrome (MDS) characterized by deletions in chromosome 5q. Studies show that haploinsufficiency of RPS14 in hematopoietic stem cells disrupts erythroid differentiation, contributing to anemia—a hallmark of this disorder. This discovery positioned RPS14 as both a molecular marker and a therapeutic target for 5q- syndrome.
RPS14 antibodies are essential tools for detecting protein expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. They help elucidate RPS14's subcellular localization (primarily nucleolar and cytoplasmic) and interactions with other ribosome-associated proteins. Recent research also explores RPS14's potential roles beyond ribosome function, including its involvement in p53 activation under ribosomal stress and possible connections to cancer progression. Commercially available RPS14 antibodies are typically validated for specificity across human, mouse, and rat samples, with applications spanning basic molecular biology, clinical diagnostics, and drug development studies targeting ribosomal pathologies.
×
