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Rabbit Polyclonal CENPBD1 Antibody

  • 中文名: CENPBD1抗体
  • 别    名: nan
货号: IPDX10282
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenFusion protein of human CENPBD1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于CENP-B(CENPBD1)抗体的3篇参考文献的简要概括:

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1. **文献名称**: *Autoantibodies to centromere proteins: Role in diagnosis and disease association*

**作者**: Fritzler, M.J. et al.

**摘要**: 该综述探讨了抗着丝粒抗体(包括CENP-B抗体)在系统性硬化症(硬皮病)等自身免疫疾病中的诊断价值,强调其作为血清学标志物的临床意义及与疾病预后的关联。

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2. **文献名称**: *The centromere: hub of chromosomal activities*

**作者**: Pluta, A.F. et al.

**摘要**: 研究解析了CENP-B蛋白在着丝粒组装中的功能,指出其通过结合特定的DNA序列(CENP-B box)参与维持染色体稳定性,并讨论了相关抗体的实验应用(如免疫定位研究)。

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3. **文献名称**: *CENP-B is required for de novo centromere formation in human cells*

**作者**: Sullivan, B.A. et al.

**摘要**: 通过抗体介导的蛋白质敲低实验,证明CENP-B在人类细胞中新着丝粒形成中的必要性,揭示了其在染色质重塑和细胞分裂中的关键作用。

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**备注**:CENP-B是着丝粒核心蛋白,其抗体研究多集中于自身免疫疾病诊断(如硬皮病)及染色体动力学机制。若需更近期文献,可补充结构生物学或疾病特异性研究(如2020年后发表)。

背景信息

The CENP-B (Centromere Protein B) antibody is a significant tool in studying centromere biology and autoimmune disorders. CENP-B is a highly conserved protein involved in centromere assembly and chromosome segregation during cell division. It binds to centromeric α-satellite DNA through a 17-bp motif (CENP-B box), facilitating kinetochore formation and ensuring proper chromosome alignment. Antibodies targeting CENP-B are frequently detected in autoimmune conditions, particularly in a subset of patients with systemic sclerosis (scleroderma) and related disorders, where they serve as diagnostic biomarkers. These autoantibodies are associated with limited cutaneous involvement and a lower risk of severe organ complications. In research, CENP-B antibodies are used to investigate centromere structure, epigenetic regulation, and mitotic mechanisms. Commercial CENP-B antibodies are generated using immunogens from conserved regions of the protein (e.g., N-terminal DNA-binding domain) and validated in applications like immunofluorescence, Western blotting, and chromatin immunoprecipitation. Their specificity helps distinguish CENP-B from other centromere proteins (e.g., CENP-A, CENP-C) in functional studies. Understanding CENP-B antibody interactions also contributes to exploring autoimmune pathogenesis and developing targeted therapies.

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